AG11546
Smooth muscle from Vein, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Vein, iliac
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Cell Type
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Smooth muscle
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Tissue Type
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Vein
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Vein, iliac
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
57 |
| PDL at Freeze |
26 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line HIVS-121T. The iliac vein was removed from a braindead, heartbeating cadaver, organ donor on 8/30/89. The donor died from a gun shot wound and had no known diseases. The culture was initiated by explantation of tissue dissected fromthe subendothelial layer. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay). The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. |
| Mesri M, Wall NR, Li J, Kim RW, Altieri DC, Cancer gene therapy using a survivin mutant adenovirus. J Clin Invest108(7):981-90 2001 |
| PubMed ID: 11581299 |
| PDL at Senescence |
57 |
| Cumulative PDL at Freeze |
26 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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