AG11182
Endothelial from Vein, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Vein, iliac
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Cell Type
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Endothelial
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Tissue Type
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Vein
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Transformant
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Untransformed
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Sample Source
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Endothelial from Vein, iliac
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.91 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line HIVE-35. The iliac vein was removed from a braindead, heartbeating cadaver, organ donor on 9/24/84. The donor died of head trauma and had no known diseases. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. Most cells were positive by immunofluorescence assay for endothelial cellspecific von Willebrand factor. Some cells were positive for alpha-smooth muscle actin (1% at PDL 19 and 1% at PDL 43). The culture was grown on on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml crude ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. |
| Nichols WW, Buynak EB, Bradt C, Hill R, Aronson M, Jarrell BE, Mueller SN, Levine EM, Cytogenetic evaluation of human endothelial cell cultures. J Cell Physiol132:453-62 1987 |
| PubMed ID: 3654763 |
| Cumulative PDL at Freeze |
22.9 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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