AG11132
Epithelial from Breast organoid
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Breast organoid
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Cell Type
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Epithelial
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Tissue Type
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Breast
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Transformant
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Untransformed
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Sample Source
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Epithelial from Breast organoid
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
59 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Clinically unremarkable breast tissue was obtained in 1978 during reduction mammoplasty. Mammary epithelial clumps or organoids were isolated by tissue dissection followed by partial enzymatic digestion with collagenase and hyaluronidase. Epithelial organoids were separated from the digested stromal elements by filtration and stored in liquid nitrogen. The culture was initiated 7/88 from thawed organoids by explantation in serum-free MCDB 170 plus supplements. Serial subcultivation resulted in the selection of a population of proliferative epithelial cells. Culture submitted as line 48R/AC170 at passage 8, PDL 30 (estimated by submitter), and has been frozen at the Repository at PDL 45. Culture is grown in serum-free medium. |
| Kim B, Pena CD, Auguste DT, Targeted Lipid Nanoemulsions Encapsulating Epigenetic Drugs Exhibit Selective Cytotoxicity on CDH1 Molecular pharmaceutics16:1813-1826 2019 |
| PubMed ID: 30883132 |
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| Kluz PN, Kolb R, Xie Q, Borcherding N, Liu Q, Luo Y, Kim MC, Wang L, Zhang Y, Li W, Stipp C, Gibson-Corley KN, Zhao C, Qi HH, Bellizzi A, Tao AW, Sugg S, Weigel RJ, Zhou D, Shen X, Zhang W, Cancer cell-intrinsic function of CD177 in attenuating ß-catenin signaling Oncogene16:1813-1826 2019 |
| PubMed ID: 32042113 |
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| Guo X, Wu Y, Hartley RS, Cold-inducible RNA-binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancer Molecular carcinogenesis49:130-40 2009 |
| PubMed ID: 19777567 |
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| Bean GR, Scott V, Yee L, Ratliff-Daniel B, Troch MM, Seo P, Bowie ML, Marcom PK, Slade J, Kimler BF, Fabian CJ, Zalles CM, Broadwater G, Baker JC, Wilke LG, Seewaldt VL, Retinoic acid receptor-beta2 promoter methylation in random periareolar fine needle aspiration Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology14:790-8 2005 |
| PubMed ID: 15824145 |
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| Dietze EC, Bowie ML, Mrózek K, Caldwell LE, Neal C, Marjoram RJ, Troch MM, Bean GR, Yokoyama KK, Ibarra CA, Seewaldt VL, CREB-binding protein regulates apoptosis and growth of HMECs grown in reconstituted ECM via laminin-5 Journal of cell science118:5005-22 2005 |
| PubMed ID: 16219677 |
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| Bowie ML, Dietze EC, Delrow J, Bean GR, Troch MM, Marjoram RJ, Seewaldt VL, Interferon-regulatory factor-1 is critical for tamoxifen-mediated apoptosis in human mammary epithelial cells Oncogene23:8743-55 2004 |
| PubMed ID: 15467738 |
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| Dietze EC, Troch MM, Bean GR, Heffner JB, Bowie ML, Rosenberg P, Ratliff B, Seewaldt VL, Tamoxifen and tamoxifen ethyl bromide induce apoptosis in acutely damaged mammary epithelial cells through modulation of AKT activity Oncogene23:3851-62 2004 |
| PubMed ID: 14990993 |
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| Yee LD, Guo Y, Bradbury J, Suster S, Clinton SK, Seewaldt VL, The antiproliferative effects of PPARgamma ligands in normal human mammary epithelial cells Breast cancer research and treatment78:179-92 2003 |
| PubMed ID: 12725418 |
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| Chen LI, Sommer KM, Swisshelm K, Downstream codons in the retinoic acid receptor beta -2 and beta -4 mRNAs
initiate translation of a protein isoform that disrupts retinoid-activated
transcription. J Biol Chem277(38):35411-21 2002 |
| PubMed ID: 12118004 |
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| Dietze EC, Caldwell LE, Grupin SL, Mancini M, Seewaldt VL, Tamoxifen but not 4-hydroxytamoxifen initiates apoptosis in p53(-) normal human mammary epithelial cells by inducing mitochondrial depolarization. J Biol Chem276(7):5384-94 2001 |
| PubMed ID: 11094056 |
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| Seewaldt VL, Mrozek K, Dietze EC, Parker M, Caldwell LE, Human papillomavirus type 16 E6 inactivation of p53 in normal human mammary epithelial cells promotes tamoxifen-mediated apoptosis. Cancer Res61(2):616-24 2001 |
| PubMed ID: 11212259 |
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| Zhang Y, Rishi AK, Dawson MI, Tschang R, Farhana L, Boyanapalli M, Reichert U,
Shroot B, Van Buren EC, Fontana JA, S-phase arrest and apoptosis induced in normal mammary epithelial cells by a
novel retinoid. Cancer Res60(7):2025-32 2000 |
| PubMed ID: 10766194 |
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| Seewaldt VL, Dietze EC, Johnson BS, Collins SJ, Parker MB, Retinoic acid-mediated G1-S-phase arrest of normal human mammary epithelial
cells is independent of the level of p53 protein expression. Cell Growth Differ10(1):49-59 1999 |
| PubMed ID: 9950218 |
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| Sommer KM, Chen LI, Treuting PM, Smith LT, Swisshelm K, Elevated retinoic acid receptor beta(4) protein in human breast tumor cells with nuclear and cytoplasmic localization. Proc Natl Acad Sci U S A96:8651-6 1999 |
| PubMed ID: 10411930 |
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| Stampfer, Isolation and growth of human mammary epithelial cells. J Tissue Culture Methods9:107 (1985):8651-6 1985 |
| PubMed ID: 10411930 |
| PDL at Senescence |
59 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEGM-mammary epithelial cell medium plus supplements |
| Serum |
0% fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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