AG10076
Fibroblast from Lung, Lung
Description:
MRC-5 - SV40 VIRUS-TRANSFORMED FETAL LUNG FIBROBLAST
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Specially Characterized Fibroblasts |
|
Biopsy Source
|
Lung
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Lung
|
|
Transformant
|
Simian Virus 40
|
|
Sample Source
|
Fibroblast from Lung, Lung
|
|
Race
|
White
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Freeze |
5.94 |
| Passage Frozen |
41 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line 5965C-VA1 is a post-crisis SV40 virus-transformed derivative of MRC-5 (AG05965C) that was derived 10/87 by infecting MRC-5 cells at PDL 27 with SV40 virus. Foci of transformed cells appeared and were scored approximately 28 days post-infection. Crisis occurred 50 PDLs post-infection. The culture was frozen at passage 38. |
| Sarmini L, Meabed M, Emmanouil E, Atsaves G, Robeska E, Karwowski BT, Campalans A, Gimisis T, Khobta A, Requirement of transcription-coupled nucleotide excision repair for the removal of a specific type of oxidatively induced DNA damage Nucleic acids research: 2023 |
| PubMed ID: 37026475 |
| |
| Kitsera N, Gasteiger K, Lühnsdorf B, Allgayer J, Epe B, Carell T, Khobta A, Cockayne syndrome: varied requirement of transcription-coupled nucleotide excision repair for the removal of three structurally different adducts from transcribed DNA PloS one9:e94405 2014 |
| PubMed ID: 24713864 |
| |
| Castillo P, Bogliolo M, Surralles J, Coordinated action of the Fanconi anemia and ataxia telangiectasia pathways in response to oxidative damage DNA repair10:518-25 2010 |
| PubMed ID: 21466974 |
| |
| Bolderson E, Scorah J, Helleday T, Smythe C, Meuth M, ATM is required for the cellular response to thymidine induced replication fork stress. Hum Mol Genet13(23):2937-45 2004 |
| PubMed ID: 15459181 |
| |
| Stavropoulos DJ, Bradshaw PS, Li X, Pasic I, Truong K, Ikura M, Ungrin M, Meyn
MS, The Bloom syndrome helicase BLM interacts with TRF2 in ALT cells and promotes
telomeric DNA synthesis. Hum Mol Genet11(25):3135-44 2002 |
| PubMed ID: 12444098 |
| |
| Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995 |
| PubMed ID: 7559606 |
| Cumulative PDL at Freeze |
100 |
| Passage Frozen |
41 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
|
|