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AG10076 Fibroblast from Lung, Lung

Description:

MRC-5 - SV40 VIRUS-TRANSFORMED FETAL LUNG FIBROBLAST

Affected:

No

Sex:

Male

Age:

14 FW (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection Specially Characterized Fibroblasts
Biopsy Source Lung
Cell Type Fibroblast
Tissue Type Lung
Transformant Simian Virus 40
Sample Source Fibroblast from Lung, Lung
Race White
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks Line 5965C-VA1 is a post-crisis SV40 virus-transformed derivative of MRC-5 (AG05965C) that was derived 10/87 by infecting MRC-5 cells at PDL 27 with SV40 virus. Foci of transformed cells appeared and were scored approximately 28 days post-infection. Crisis occurred 50 PDLs post-infection. The culture was frozen at passage 38.

Characterizations

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PDL at Freeze 5.94
Passage Frozen 41
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 

Phenotypic Data

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Remarks Line 5965C-VA1 is a post-crisis SV40 virus-transformed derivative of MRC-5 (AG05965C) that was derived 10/87 by infecting MRC-5 cells at PDL 27 with SV40 virus. Foci of transformed cells appeared and were scored approximately 28 days post-infection. Crisis occurred 50 PDLs post-infection. The culture was frozen at passage 38.

Publications

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Sarmini L, Meabed M, Emmanouil E, Atsaves G, Robeska E, Karwowski BT, Campalans A, Gimisis T, Khobta A, Requirement of transcription-coupled nucleotide excision repair for the removal of a specific type of oxidatively induced DNA damage Nucleic acids research: 2023
PubMed ID: 37026475
 
Kitsera N, Gasteiger K, Lühnsdorf B, Allgayer J, Epe B, Carell T, Khobta A, Cockayne syndrome: varied requirement of transcription-coupled nucleotide excision repair for the removal of three structurally different adducts from transcribed DNA PloS one9:e94405 2014
PubMed ID: 24713864
 
Castillo P, Bogliolo M, Surralles J, Coordinated action of the Fanconi anemia and ataxia telangiectasia pathways in response to oxidative damage DNA repair10:518-25 2010
PubMed ID: 21466974
 
Bolderson E, Scorah J, Helleday T, Smythe C, Meuth M, ATM is required for the cellular response to thymidine induced replication fork stress. Hum Mol Genet13(23):2937-45 2004
PubMed ID: 15459181
 
Stavropoulos DJ, Bradshaw PS, Li X, Pasic I, Truong K, Ikura M, Ungrin M, Meyn MS, The Bloom syndrome helicase BLM interacts with TRF2 in ALT cells and promotes telomeric DNA synthesis. Hum Mol Genet11(25):3135-44 2002
PubMed ID: 12444098
 
Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995
PubMed ID: 7559606

External Links

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dbSNP dbSNP ID: 22176

Culture Protocols

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Cumulative PDL at Freeze 100
Passage Frozen 41
Split Ratio 1:6
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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