AG09799
Endothelial from Aorta, abdominal
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Aorta, abdominal
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, abdominal
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY/46,XY,-13,+mar/46,XY,-13,+frag
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
29 |
| Passage Frozen |
13 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line HAAE-1. The abdominal aorta was removed from a braindead, heartbeating cadaver, renal donor on 2/07/83. The donor died of head trauma and had no known diseases. The culture was initiated on 2/08/83 using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. The culture is a mosaic with karyotype: 46,XY/46,XY,-13,+mar/46,XY, -13,+frag; unbalanced; 10%/50%/40% with 6% having a +11. The marker may be a del(13). This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Elkhodiry MA, Boulanger MD, Bashth O, Tanguay JF, Laroche G, Hoesli CA, Isolating and expanding endothelial progenitor cells from peripheral blood on peptide-functionalized polystyrene surfaces Biotechnology and bioengineering116:2598-2609 2019 |
| PubMed ID: 31286475 |
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| Cooper S, Emmott A, McDonald KK, Campeau MA, Leask RL, Increased MMP activity in curved geometries disrupts the endothelial cell glycocalyx creating a proinflammatory environment PloS one13:e0202526 2018 |
| PubMed ID: 30138400 |
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| Dick M, Jonak P, Leask RL, Statin therapy influences endothelial cell morphology and F-actin cytoskeleton structure when exposed to static and laminar shear stress conditions Life sciences92:859-65 2012 |
| PubMed ID: 23517776 |
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| Zhang A, Cheng TP, Altura BT, Altura BM., Mg2+ and caffeine-induced intracellular Ca2+ release in human vascular endothelial cells. Br J Pharmacol109:291-2 1993 |
| PubMed ID: 8358532 |
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| Nichols WW, Buynak EB, Bradt C, Hill R, Aronson M, Jarrell BE, Mueller SN, Levine EM, Cytogenetic evaluation of human endothelial cell cultures. J Cell Physiol132:453-62 1987 |
| PubMed ID: 3654763 |
| Cumulative PDL at Freeze |
29 |
| Passage Frozen |
13 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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