AG09605
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY/46,XY,t(15;19)(p11.2;p13.1), t(15;19)(q26.3;q13.1)/46,XY,t(4;6) (p12;q13),t(11;20)(q13;p13)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
40 |
| PDL at Freeze |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,t(15;19)(p11.2;p13.1),t(15;19)(q26.3;q13.1)/ 46,XY,t(4;6)(p12;q13),t(11;20)(q13;p13); balanced; 18%/68%/12% with 2% of the cells examined showing other abnormalities. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Bersini S, Schulte R, Huang L, Tsai H, Hetzer MW, Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome eLife9: 2019 |
| PubMed ID: 32896271 |
| PDL at Senescence |
40 |
| Cumulative PDL at Freeze |
8 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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