AG09429
Fibroblast from Gingival, Gum
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Gum
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Cell Type
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Fibroblast
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Tissue Type
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Gingival
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Gingival, Gum
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.8 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The culture was initiated on 3/06/87 from explants of minced gum tissue removed ante-mortem. The cell morphology is fibroblast-like and the karyotype is 46,XX. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Ricci A, Biancucci F, Morganti G, Magnani M, Menotta M, New human ATM variants are able to regain ATM functions in ataxia telangiectasia disease Cellular and molecular life sciences : CMLS79:601 2022 |
| PubMed ID: 36422718 |
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| Morozzi G, Rothen J, Toussaint G, De Lange K, Westritschnig K, Doelemeyer A, Ueberschlag VP, Kahle P, Lambert C, Obrecht M, Beckmann N, Ritter V, Panesar M, Stauffer D, Garnier I, Mueller M, Guerini D, Keller CG, Knehr J, Roma G, Bidinosti M, Brachat S, Morvan F, Fornaro M, STING regulates peripheral nerve regeneration and colony stimulating factor 1 receptor (CSF1R) processing in microglia iScience24:103434 2021 |
| PubMed ID: 34877494 |
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| Ungricht R, Guibbal L, Lasbennes MC, Orsini V, Beibel M, Waldt A, Cuttat R, Carbone W, Basler A, Roma G, Nigsch F, Tchorz JS, Hoepfner D, Hoppe PS, Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis Cell stem cell29:160-175.e7 2021 |
| PubMed ID: 34847364 |
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| Ricci A, Orazi S, Biancucci F, Magnani M, Menotta M, The nucleoplasmic interactions among Lamin A/C-pRB-LAP2a-E2F1 are modulated by dexamethasone Scientific reports11:10099 2020 |
| PubMed ID: 33980953 |
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| Schrank S, McDaid J, Briggs CA, Mustaly-Kalimi S, Brinks D, Houcek A, Singer O, Bottero V, Marr RA, Stutzmann GE, Human-Induced Neurons from Presenilin 1 Mutant Patients Model Aspects of Alzheimer's Disease Pathology International journal of molecular sciences21:10099 2020 |
| PubMed ID: 32033164 |
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| Ricci A, Galluzzi L, Magnani M, Menotta M, DDIT4 gene expression is switched on by a new HDAC4 function in ataxia telangiectasia FASEB journal : official publication of the Federation of American Societies for Experimental Biology34:1802-1818 2019 |
| PubMed ID: 31914654 |
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| Stevens RH, de Moura Martins Lobo Dos Santos C, Zuanazzi D, de Accioly Mattos MB, Ferreira DF, Kachlany SC, Tinoco EM, Prophage induction in lysogenic Aggregatibacter actinomycetemcomitans cells co-cultured with human gingival fibroblasts, and its effect on leukotoxin release Microbial pathogenesis54:54-9 2012 |
| PubMed ID: 23022667 |
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| Li K, Besse EK, Ha D, Kovtunovych G, Rouault TA, Iron-dependent regulation of frataxin expression: implications for treatment of Friedreich ataxia Human molecular genetics17:2265-73 2008 |
| PubMed ID: 18424449 |
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| Argentin G, Cicchetti R, Genotoxic and antiapoptotic effect of nicotine on human gingival fibroblasts. Toxicol Sci79(1):75-81 2004 |
| PubMed ID: 14718647 |
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| Chang HY, Chi JT, Dudoit S, Bondre C, van de Rijn M, Botstein D, Brown PO, Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci U S A99(20):12877-82 2002 |
| PubMed ID: 12297622 |
| Cumulative PDL at Freeze |
9.8 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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