AG08446
Fibroblast from Skin, Arm
Description:
ALZHEIMER DISEASE; AD
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Italian Alzheimer Disease |
|
Biopsy Source
|
Arm
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Arm
|
|
Race
|
White
|
|
Ethnicity
|
ITALIAN
|
|
Family Member
|
41
|
|
Relation to Proband
|
VI-2085
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Freeze |
2 |
| Passage Frozen |
2 |
| |
| Remarks |
The donor admits to occasional problems with memory, collateral family history indicates a progressive deterioration in psychosocial function attended by an increase in intellectual impairment. Neurological exams have shown changes in his mental status characterized by disorientation to time, poor recent memory, dyscalculia, and decreased attention span. EEG and CSF exams were normal. MRI scan revealed diffuse cerebral atrophy. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 10/10/85 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 2. A lymphoblast culture from same donor is AG08445. |
| Schrank S, McDaid J, Briggs CA, Mustaly-Kalimi S, Brinks D, Houcek A, Singer O, Bottero V, Marr RA, Stutzmann GE, Human-Induced Neurons from Presenilin 1 Mutant Patients Model Aspects of Alzheimer's Disease Pathology International journal of molecular sciences21: 2020 |
| PubMed ID: 32033164 |
| |
| Querfurth HW, Wijsman EM, St George-Hyslop PH, Selkoe DJ, Beta APP mRNA transcription is increased in cultured fibroblasts from the familial Alzheimer's disease-1 family. Brain Res Mol Brain Res28(2):319-37 1995 |
| PubMed ID: 7723630 |
| |
| St George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Myers RH, Feldman RG, Pollen D, Drachman D, et al, The genetic defect causing familial Alzheimer's disease maps on chromosome 21. Science235:885-90 1987 |
| PubMed ID: 2880399 |
| |
| Foncin, Presenile Alzheimer's disease in a large kindred. Rev Neurol141:194 (1985):885-90 1985 |
| PubMed ID: 4001707 |
| Cumulative PDL at Freeze |
2 |
| Passage Frozen |
2 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|