AG08269
Fibroblast from Skin, Arm
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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7
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Relation to Proband
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paternal grandmother
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
9.04 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Remarks |
Donor is a clinically unaffected member of an Alzheimer's disease family. Donor's deceased son was affected. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 6/21/85 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 3. Donor's affected granddaughter is AG08744 Lymphoblast. A lymphoblast culture from same donor is AG08268. |
| Rossi A, Rigotto G, Valente G, Giorgio V, Basso E, Filadi R, Pizzo P, Defective Mitochondrial Pyruvate Flux Affects Cell Bioenergetics in Alzheimer's Disease-Related Models Cell reports30:2332-2348.e10 2019 |
| PubMed ID: 32075767 |
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| Mendonsa G, Dobrowolska J, Lin A, Vijairania P, Jong YJ, Baenziger NL, Molecular profiling reveals diversity of stress signal transduction cascades in highly penetrant Alzheimer's disease human skin fibroblasts PLoS ONE4:e4655 2008 |
| PubMed ID: 19247475 |
| Cumulative PDL at Freeze |
12.04 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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