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AG07090 Mesothelial from Ascites fluid

Description:

OVARIAN CANCER

Affected:

No

Sex:

Female

Age:

58 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection Apparently Healthy Collection
Biopsy Source Ascites fluid
Cell Type Mesothelial
Tissue Type Peritoneum
Transformant Untransformed
Sample Source Mesothelial from Ascites fluid
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks Normal human mesothelial cell strain LP-3. This culture was initiated on 10/1/83 by directly plating an ascites fluid cell suspension which had been stored frozen since removal from the peritoneal cavity of an ovarian cancer patient on 9/19/80. The culture medium must be supplemented with 10 nanograms/ml EGF and 0.4 micrograms/ml hydrocortisone. The cell morphology is fibroblast-like in medium supplemented with EGF, but is epithelial-like in medium without EGF. The karyotype is 46,XX; normal diploid female. **This line will only be shipped frozen.** The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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PDL at Freeze 4.59
Passage Frozen 11
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 

Phenotypic Data

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Remarks Normal human mesothelial cell strain LP-3. This culture was initiated on 10/1/83 by directly plating an ascites fluid cell suspension which had been stored frozen since removal from the peritoneal cavity of an ovarian cancer patient on 9/19/80. The culture medium must be supplemented with 10 nanograms/ml EGF and 0.4 micrograms/ml hydrocortisone. The cell morphology is fibroblast-like in medium supplemented with EGF, but is epithelial-like in medium without EGF. The karyotype is 46,XX; normal diploid female. **This line will only be shipped frozen.** The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Ren J, Xiao YJ, Singh LS, Zhao X, Zhao Z, Feng L, Rose TM, Prestwich GD, Xu Y, Lysophosphatidic acid is constitutively produced by human peritoneal mesothelial cells and enhances adhesion, migration, and invasion of ovarian cancer cells Cancer research66:3006-14 2006
PubMed ID: 16540649
 
Kanerva A, Wang M, Bauerschmitz GJ, Lam JT, Desmond RA, Bhoola SM, Barnes MN, Alvarez RD, Siegal GP, Curiel DT, Hemminki A, Gene transfer to ovarian cancer versus normal tissues with fiber-modified adenoviruses. Mol Ther5(6):695-704 2002
PubMed ID: 12027553
 
Casado E, Gomez-Navarro J, Yamamoto M, Adachi Y, Coolidge CJ, Arafat WO, Barker SD, Wang MH, Mahasreshti PJ, Hemminki A, Gonzalez-Baron M, Barnes MN, Pustilnik TB, Siegal GP, Alvarez RD, Curiel DT, Strategies to accomplish targeted expression of transgenes in ovarian cancer for molecular therapeutic applications. Clin Cancer Res7(8):2496-504 2001
PubMed ID: 11489832
 
Lee TC, Zhang Y, Aston C, Hintz R, Jagirdar J, Perle MA, Burt M, Rom WN, Normal human mesothelial cells and mesothelioma cell lines express insulin-like growth factor I and associated molecules. Cancer Res53:2858-64 1993
PubMed ID: 7684950

External Links

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dbSNP dbSNP ID: 19334

Culture Protocols

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Cumulative PDL at Freeze 29.59
Passage Frozen 11
Split Ratio 1:6
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Medium 199 in Earle BSS/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Supplement Epidermal Growth Factor 10 ng/ml
Supplement Hydrocortisone 0.4 ug/ml
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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How to Order
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