AG06308
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY{64}/46,XY,t(13;15)(q14;q12){36}
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
47 |
| PDL at Freeze |
13 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. Culture is a mosaic with karyotype: 46,XY/46,XY,t(13;15)(q14;q12); 64%/36%; with 18% of the cells examined showing random chromosome loss and 2% showing random chromosomal aberrations. A culture initiated from a biopsy taken 2 years later from this same donor is AG07725A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
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| Reed MJ, Bradshaw AD, Shaw M, Sadoun E, Han N, Ferara N, Funk S, Puolakkainen P, Sage EH, Enhanced angiogenesis characteristic of SPARC-null mice disappears with age Journal of cellular physiology204:800-7 2005 |
| PubMed ID: 15795937 |
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| Reed, TGF-beta 1 induces the expression of type 1 collagen and SPARC, and enhances contraction of collagen gels, by fibroblasts from young and aged donors. J Cell Biol158:169 (1994):800-7 1994 |
| PubMed ID: 15795937 |
| PDL at Senescence |
47 |
| Cumulative PDL at Freeze |
13 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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