AG06173
Fibroblast from Lung, Lung
Description:
TIG-1 - NORMAL HUMAN FETAL LUNG FIBROBLAST
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Specially Characterized Fibroblasts |
|
Biopsy Source
|
Lung
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Lung
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Lung, Lung
|
|
Race
|
Asian
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XX
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
67 |
| PDL at Freeze |
17 |
| Passage Frozen |
9 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
The TIG-1 cell line was initiated at the Tokyo Metropolitan Institute of Gerontology on 7/28/76 using lung tissue from a 5 month gestational age fetus from a therapeutic abortion. Cells released by trypsin digestion of a lung specimen were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. The maximum number of population doublings achieved to senescence range from 55 to 79. G6PD is isoenzyme type B. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Ye Q, Zhang Y, Cao Y, Wang X, Guo Y, Chen J, Horn J, Ponomareva LV, Chaiswing L, Shaaban KA, Wei Q, Anderson BD, St Clair DK, Zhu H, Leggas M, Thorson JS, She QB, Frenolicin B Targets Peroxiredoxin 1 and Glutaredoxin 3 to Trigger ROS/4E-BP1-Mediated Antitumor Effects Cell chemical biology: 2018 |
| PubMed ID: 30661989 |
| |
| Clemson CM, Hall LL, Byron M, McNeil J, Lawrence JB, The X chromosome is organized into a gene-rich outer rim and an internal core containing silenced nongenic sequences Proceedings of the National Academy of Sciences of the United States of America103:7688-93 2006 |
| PubMed ID: 16682630 |
| |
| Smith KP, Byron M, Clemson CM, Lawrence JB, Ubiquitinated proteins including uH2A on the human and mouse inactive X chromosome: enrichment in gene rich bands Chromosoma113:324-35 2004 |
| PubMed ID: 15616869 |
| |
| Ohashi M, Aizawa S, Ooka H, Ohsawa T, Kaji K, Kondo H, Kobayashi T, Noumura T, Matsuo M, Mitsui Y, Murota S, Yamamoto K, Ito H, Shimada H, Utakoji T, A new human diploid cell strain, TIG-1, for the research on cellular aging. Exp Gerontol15:121-33 1980 |
| PubMed ID: 7389832 |
| PDL at Senescence |
67 |
| Cumulative PDL at Freeze |
17 |
| Passage Frozen |
9 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|