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AG06010 Fibroblast from Skin, Arm

Description:

GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)

Affected:

No

Sex:

Female

Age:

62 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Images
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection GRC
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Arm
Race White
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XX
Species Homo sapiens
Common Name Human
Remarks The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XX; diploid female. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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PDL at Freeze 14
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 

Phenotypic Data

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Remarks The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XX; diploid female. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Kamienieva I, Charzynska A, Duszynski J, Malinska D, Szczepanowska J, In search for mitochondrial biomarkers of Parkinson's disease: Findings in parkin-mutant human fibroblasts Biochimica et biophysica acta Molecular basis of disease1869:166787 2023
PubMed ID: 37302428
 
Thomas R, Moloney EB, Macbain ZK, Hallett PJ, Isacson O, Fibroblasts from idiopathic Parkinson's disease exhibit deficiency of lysosomal glucocerebrosidase activity associated with reduced levels of the trafficking receptor LIMP2 Molecular brain14:16 2020
PubMed ID: 33468204
 
Mazzola JL, Sirover MA, Subcellular alteration of glyceraldehyde-3-phosphate dehydrogenase in Alzheimer's disease fibroblasts Journal of neuroscience research71:279-85 2002
PubMed ID: 12503091
 
Mazzola JL, Sirover MA, Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts. J Neurochem76(2):442-9 2001
PubMed ID: 11208907
 
Etcheberrigaray E, Gibson GE, Alkon DL, Molecular mechanisms of memory and the pathophysiology of Alzheimer's disease. Ann N Y Acad Sci747:245-55 1994
PubMed ID: 7847674
 
Ito E, Oka K, Etcheberrigaray R, Nelson TJ, McPhie DL, Tofel-Grehl B, Gibson GE, Alkon DL, Internal Ca2+ mobilization is altered in fibroblasts from patients with Alzheimer disease. Proc Natl Acad Sci U S A91:534-8 1994
PubMed ID: 8290560
 
Etcheberrigaray R, Ito E, Oka K, Tofel-Grehl B, Gibson GE, Alkon DL, Potassium channel dysfunction in fibroblasts identifies patients with Alzheimer disease. Proc Natl Acad Sci U S A90:8209-13 1993
PubMed ID: 8367484
 
Huang HM, Toral-Barza L, Thaler H, Tofel-Grehl B, Gibson GE, Inositol phosphates and intracellular calcium after bradykinin stimulation in fibroblasts from young, normal aged and Alzheimer donors. Neurobiol Aging12:469-73 1991
PubMed ID: 1770982
 
Kinsella TJ, Dobson PP, Fornace AJ Jr, Barrett SF, Ganges MB, Robbins JH, Alzheimer's disease fibroblasts have normal repair of N-methyl-N'-nitro- N-nitrosoguanidine-induced DNA damage determined by the alkaline elution technique. Biochem Biophys Res Commun149:355-61 1987
PubMed ID: 3426578
 
Sims NR, Finegan JM, Blass JP, Altered metabolic properties of cultured skin fibroblasts in Alzheimer's disease. Ann Neurol21:451-7 1987
PubMed ID: 3109316
 
Li JC, Kaminskas E, Deficient repair of DNA lesions in Alzheimer's disease fibroblasts. Biochem Biophys Res Commun129:733-8 1985
PubMed ID: 2409969
 
Sims NR, Finegan JM, Blass JP, Altered glucose metabolism in fibroblasts from patients with Alzheimer's disease [letter] N Engl J Med313:638-9 1985
PubMed ID: 4022053

External Links

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dbSNP dbSNP ID: 22579

Images

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View karyotype 

Culture Protocols

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Cumulative PDL at Freeze 14
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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