AG05965-D
Fibroblast from Lung, Lung
Description:
MRC-5 - NORMAL HUMAN FETAL LUNG FIBROBLAST
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Specially Characterized Fibroblasts |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
48 |
| PDL at Freeze |
40 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The MRC-5 cell line was developed in September 1966 from lung tissue taken from a 14 week fetus aborted for psychiatric reason from a 27 year old physically healthy woman. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. Cumulative population doublings to senescence is 42-48. G6PD isoenzyme is type B. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Bots ST, Kemp V, Cramer SJ, van den Wollenberg DJ, Hornsveld M, Lamfers M, van der Pluijm G, Hoeben RC, Non-human primate adenoviruses of the Human Adenovirus B species are potent and broadly-acting oncolytic vector candidates Human gene therapy: 2021 |
| PubMed ID: 34861769 |
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| Birar VC, Sheerin AN, Ostler EL, Faragher RGA, Novel resveratrol derivatives have diverse effects on the survival, proliferation and senescence of primary human fibroblasts Biogerontology: 2020 |
| PubMed ID: 32793997 |
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| Jühlen R, Martinelli V, Vinci C, Breckpot J, Fahrenkrog B, Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts Scientific reports10:19301 2020 |
| PubMed ID: 33168876 |
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| Gräb J, Suárez I, van Gumpel E, Winter S, Schreiber F, Esser A, Hölscher C, Fritsch M, Herb M, Schramm M, Wachsmuth L, Pallasch C, Pasparakis M, Kashkar H, Rybniker J, Corticosteroids inhibit Mycobacterium tuberculosis-induced necrotic host cell death by abrogating mitochondrial membrane permeability transition Nature communications10:688 2018 |
| PubMed ID: 30737374 |
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| Guo WJ, Datta S, Band V, Dimri GP, Mel-18, a polycomb group protein, regulates cell proliferation and senescence via transcriptional repression of Bmi-1 and c-Myc oncoproteins Molecular biology of the cell18:536-46 2006 |
| PubMed ID: 17151361 |
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| Skandalis A, Uribe E, A survey of splice variants of the human hypoxanthine phosphoribosyl transferase and DNA polymerase beta genes: products of alternative or aberrant splicing? Nucleic Acids Res32(22):6557-64 2004 |
| PubMed ID: 15601998 |
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| Von Kobbe C, May A, Grandori C, Bohr VA, Werner syndrome cells escape hydrogen peroxide-induced cell proliferation arrest. FASEB J18(15):1970-2 2004 |
| PubMed ID: 15459124 |
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| Hande MP, Balajee AS, Tchirkov A, Wynshaw-Boris A, Lansdorp PM, Extra-chromosomal telomeric DNA in cells from Atm(-/-) mice and patients with ataxia-telangiectasia. Hum Mol Genet10(5):519-28 2001 |
| PubMed ID: 11181576 |
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| Gyulai Z, Endresz V, Burian K, Pincus S, Toldy J, Cox WI, Meric C, Plotkin S, Gonczol E, Berencsi K, Cytotoxic T lymphocyte (CTL) responses to human cytomegalovirus pp65, IE1-Exon4, gB, pp150, and pp28 in healthy individuals: reevaluation of prevalence of IE1-specific CTLs. J Infect Dis181(5):1537-46 2000 |
| PubMed ID: 10823751 |
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| Piret B, Schoonbroodt S, Piette J, The ATM protein is required for sustained activation of NF-kappaB following DNA damage. Oncogene18(13):2261-71 1999 |
| PubMed ID: 10327072 |
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| Jongmans W, Vuillaume M, Chrzanowska K, Smeets D, Sperling K, Hall J, Nijmegen breakage syndrome cells fail to induce the p53-mediated DNA damage response following exposure to ionizing radiation. Mol Cell Biol17(9):5016-22 1997 |
| PubMed ID: 9271379 |
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| Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995 |
| PubMed ID: 7534307 |
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| Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995 |
| PubMed ID: 7559606 |
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| Futreal PA, Cochran C, Marks JR, Iglehart JD, Zimmerman W, Barrett JC, Wiseman RW, Mutation analysis of the THRA1 gene in breast cancer: deletion/fusion of the gene to a novel sequence on 17q in the BT474 cell line. Cancer Res54:1791-4 1994 |
| PubMed ID: 7511052 |
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| Kilfeather SA, Collins D, McCormack P, Cotter T, O'Malley K, The effect of in vitro aging on human lung fibroblast beta-adrenergic receptor density, coupling and response. Mech Ageing Dev63:247-56 1992 |
| PubMed ID: 1319530 |
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| Jacobs, Characteristics of a human diploid cell designated MRC-5. Nature277:168 (1970):247-56 1970 |
| PubMed ID: 1319530 |
| PDL at Senescence |
48 |
| Cumulative PDL at Freeze |
40 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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