AG05356
Fibroblast from Skin, Thigh
Description:
LAGOTHRIX LAGOTRICHA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thigh
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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62,XX
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Species
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Lagothrix lagotricha
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Common Name
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common woolly monkey
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Remarks
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| PDL at Freeze |
16 |
| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Genbank |
COX1 |
| Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008 |
| PubMed ID: 18799582 |
| |
| Ray DA, Xing J, Hedges DJ, Hall MA, Laborde ME, Anders BA, White BR, Stoilova N, Fowlkes JD, Landry KE, Chemnick LG, Ryder OA, Batzer MA, Alu insertion loci and platyrrhine primate phylogeny. Mol Phylogenet Evol35(1):117-26 2005 |
| PubMed ID: 15737586 |
| |
| Sawyer SL, Wu LI, Emerman M, Malik HS, Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A102(8):2832-7 2005 |
| PubMed ID: 15689398 |
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| Ruiz-Herrera A, García F, Mora L, Egozcue J, Ponsà M, Garcia M, Evolutionary conserved chromosomal segments in the human karyotype are bounded by unstable chromosome bands Cytogenetic and genome research108:161-74 2003 |
| PubMed ID: 15545726 |
| Cumulative PDL at Freeze |
16 |
| Passage Frozen |
7 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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