AG05025
Fibroblast from Skin, Skin
Description:
TRIPLOID CHROMOSOME NUMBER
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Race
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White
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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69,XXY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
The donor was born prematurely with multiple congenital abnormalities typical for triploidy. The culture was initiated on 5/20/81 using explants of minced skin tissue obtained post-mortem. The karyotype is 69,XXY. The cell morphology is fibroblast-like. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Kratka C, Vadapalli PS, Mendola R, Garrisi J, Xu J, Treff NR, Marin D, Accurate detection and frequency of abnormal ploidy in the human blastocyst F&S science: 2022 |
| PubMed ID: 36863445 |
| Cumulative PDL at Freeze |
5 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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