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AG04525 Fibroblast from Skin, Buttock

Description:

APPARENTLY HEALTHY FETAL TISSUE

Affected:

No

Sex:

Male

Age:

17 FW (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection Apparently Healthy Collection
Biopsy Source Buttock
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Buttock
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XY
Species Homo sapiens
Common Name Human
Remarks The culture was initiated on 5/11/78 from explants of minced skin tissue taken from a therapeutically aborted fetus of 17 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. A matched lung-derived fibroblast culture from same fetus is AG04526. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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PDL at Freeze 17
Passage Frozen 6
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 

Phenotypic Data

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Remarks The culture was initiated on 5/11/78 from explants of minced skin tissue taken from a therapeutically aborted fetus of 17 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. A matched lung-derived fibroblast culture from same fetus is AG04526. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Lan YY, Heather JM, Eisenhaure T, Garris CS, Lieb D, Raychowdhury R, Hacohen N, Extranuclear DNA accumulates in aged cells and contributes to senescence and inflammation Aging cell:e12901 2018
PubMed ID: 30706626
 
Russanova VR, Hirai TH, Howard BH, Semirandom sampling to detect differentiation-related and age-related epigenome remodeling The journals of gerontology Series A, Biological sciences and medical sciences59:1221-33 2005
PubMed ID: 15699521
 
Russanova VR, Hirai TH, Tchernov AV, Howard BH, Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach The journals of gerontology Series A, Biological sciences and medical sciences59:1234-43 2005
PubMed ID: 15699522
 
Chang HY, Chi JT, Dudoit S, Bondre C, van de Rijn M, Botstein D, Brown PO, Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci U S A99(20):12877-82 2002
PubMed ID: 12297622
 
Cristofalo VJ, Allen RG, Pignolo RJ, Martin BG, Beck JC, Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation. Proc Natl Acad Sci U S A95:10614-9 1998
PubMed ID: 9724752
 
Allen RG, Keogh BP, Gerhard GS, Pignolo R, Horton J, Cristofalo VJ, Expression and regulation of superoxide dismutase activity in human skin fibroblasts from donors of different ages. J Cell Physiol165:576-87 1995
PubMed ID: 7593237
 
Romanus JA, Tseng LY, Yang YW, Rechler MM, The 34 kilodalton insulin-like growth factor binding proteins in human cerebrospinal fluid and the A673 rhabdomyosarcoma cell line are human homologues of the rat BRL-3A binding protein. Biochem Biophys Res Commun163:875-81 1989
PubMed ID: 2476983
 
Conover CA, Rosenfeld RG, Hintz RL, Insulin-like growth factor II binding and action in human fetal fibroblasts. J Cell Physiol133:560-6 1987
PubMed ID: 2961773
 
Clemmons DR, Elgin RG, Han VK, Casella SJ, D'Ercole AJ, Van Wyk JJ, Cultured fibroblast monolayers secrete a protein that alters the cellular binding of somatomedin-C/insulinlike growth factor I. J Clin Invest77:1548-56 1986
PubMed ID: 2422210
 
Conover CA, Rosenfeld RG, Hintz RL, Hormonal control of the replication of human fetal fibroblasts: role of somatomedin C/insulin-like growth factor I. J Cell Physiol128:47-54 1986
PubMed ID: 3013906
 
Wilhelm, Human fibroblast collagenase: Glycosylat ion and tissue-specific levels of enzyme synthesis. Proc Natl Acad Sci USA83:3756 (1986):47-54 1986
PubMed ID: 3013906
 
Puvion-Dutilleul F, Macieira-Coelho A, Aging dependent nucleolar and chromatin changes in cultivated fibroblasts. Cell Biol Int Rep7:61-71 1983
PubMed ID: 6831570

External Links

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dbSNP dbSNP ID: 20397

Culture Protocols

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Cumulative PDL at Freeze 17
Passage Frozen 6
Split Ratio 1:1
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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