AG04525
Fibroblast from Skin, Buttock
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Buttock
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Buttock
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
17 |
| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The culture was initiated on 5/11/78 from explants of minced skin tissue taken from a therapeutically aborted fetus of 17 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. A matched lung-derived fibroblast culture from same fetus is AG04526. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Lan YY, Heather JM, Eisenhaure T, Garris CS, Lieb D, Raychowdhury R, Hacohen N, Extranuclear DNA accumulates in aged cells and contributes to senescence and inflammation Aging cell:e12901 2018 |
| PubMed ID: 30706626 |
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| Russanova VR, Hirai TH, Howard BH, Semirandom sampling to detect differentiation-related and age-related epigenome remodeling The journals of gerontology Series A, Biological sciences and medical sciences59:1221-33 2005 |
| PubMed ID: 15699521 |
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| Russanova VR, Hirai TH, Tchernov AV, Howard BH, Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach The journals of gerontology Series A, Biological sciences and medical sciences59:1234-43 2005 |
| PubMed ID: 15699522 |
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| Chang HY, Chi JT, Dudoit S, Bondre C, van de Rijn M, Botstein D, Brown PO, Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci U S A99(20):12877-82 2002 |
| PubMed ID: 12297622 |
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| Cristofalo VJ, Allen RG, Pignolo RJ, Martin BG, Beck JC, Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation. Proc Natl Acad Sci U S A95:10614-9 1998 |
| PubMed ID: 9724752 |
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| Allen RG, Keogh BP, Gerhard GS, Pignolo R, Horton J, Cristofalo VJ, Expression and regulation of superoxide dismutase activity in human skin fibroblasts from donors of different ages. J Cell Physiol165:576-87 1995 |
| PubMed ID: 7593237 |
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| Romanus JA, Tseng LY, Yang YW, Rechler MM, The 34 kilodalton insulin-like growth factor binding proteins in human cerebrospinal fluid and the A673 rhabdomyosarcoma cell line are human homologues of the rat BRL-3A binding protein. Biochem Biophys Res Commun163:875-81 1989 |
| PubMed ID: 2476983 |
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| Conover CA, Rosenfeld RG, Hintz RL, Insulin-like growth factor II binding and action in human fetal fibroblasts. J Cell Physiol133:560-6 1987 |
| PubMed ID: 2961773 |
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| Clemmons DR, Elgin RG, Han VK, Casella SJ, D'Ercole AJ, Van Wyk JJ, Cultured fibroblast monolayers secrete a protein that alters the cellular binding of somatomedin-C/insulinlike growth factor I. J Clin Invest77:1548-56 1986 |
| PubMed ID: 2422210 |
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| Conover CA, Rosenfeld RG, Hintz RL, Hormonal control of the replication of human fetal fibroblasts: role of somatomedin C/insulin-like growth factor I. J Cell Physiol128:47-54 1986 |
| PubMed ID: 3013906 |
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| Wilhelm, Human fibroblast collagenase: Glycosylat ion and tissue-specific levels of enzyme synthesis. Proc Natl Acad Sci USA83:3756 (1986):47-54 1986 |
| PubMed ID: 3013906 |
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| Puvion-Dutilleul F, Macieira-Coelho A, Aging dependent nucleolar and chromatin changes in cultivated fibroblasts. Cell Biol Int Rep7:61-71 1983 |
| PubMed ID: 6831570 |
| Cumulative PDL at Freeze |
17 |
| Passage Frozen |
6 |
| Split Ratio |
1:1 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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