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AG04452 Fibroblast from Lung, Lung

Description:

APPARENTLY HEALTHY FETAL TISSUE

Affected:

No

Sex:

Female

Age:

16 FW (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection Apparently Healthy Collection
Biopsy Source Lung
Cell Type Fibroblast
Tissue Type Lung
Transformant Untransformed
Sample Source Fibroblast from Lung, Lung
Race Black/African American
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XX
Species Homo sapiens
Common Name Human
Remarks The culture was initiated on 9/2/80 from explants of minced lung tissue taken from a therapeutically aborted fetus of 16 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A matched skin-derived fibroblast culture from same fetus is AG04451. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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PDL at Senescence 29
PDL at Freeze 11
Passage Frozen 3
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 

Phenotypic Data

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Remarks The culture was initiated on 9/2/80 from explants of minced lung tissue taken from a therapeutically aborted fetus of 16 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A matched skin-derived fibroblast culture from same fetus is AG04451. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Zhu B, Fisher E, Li L, Zhong P, Yan Z, Feng J, PTBP2 attenuation facilitates fibroblast to neuron conversion by promoting alternative splicing of neuronal genes Stem cell reports18:2268-2282 2022
PubMed ID: 37832540

External Links

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dbSNP dbSNP ID: 22197

Culture Protocols

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PDL at Senescence 29
Cumulative PDL at Freeze 11
Passage Frozen 3
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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