AG04392
Fibroblast from Skin, Buttock-thigh
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Buttock-thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Buttock-thigh
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
0 |
| PDL at Freeze |
17 |
| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The culture was initiated on 6/25/79 from explants of minced skin tissue taken from a therapeutically aborted fetus of 16 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A matched lung-derived fibroblast culture from same fetus is AG04393. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Krivega M, Stiefel CM, Karbassi S, Andersen LL, Chunduri NK, Donnelly N, Pichlmair A, Storchová Z, Genotoxic stress in constitutive trisomies induces autophagy and the innate immune response via the cGAS-STING pathway Communications biology4:831 2021 |
| PubMed ID: 34215848 |
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| Lan YY, Heather JM, Eisenhaure T, Garris CS, Lieb D, Raychowdhury R, Hacohen N, Extranuclear DNA accumulates in aged cells and contributes to senescence and inflammation Aging cell4:e12901 2018 |
| PubMed ID: 30706626 |
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| Russanova VR, Hirai TH, Howard BH, Semirandom sampling to detect differentiation-related and age-related epigenome remodeling The journals of gerontology Series A, Biological sciences and medical sciences59:1221-33 2005 |
| PubMed ID: 15699521 |
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| Russanova VR, Hirai TH, Tchernov AV, Howard BH, Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach The journals of gerontology Series A, Biological sciences and medical sciences59:1234-43 2005 |
| PubMed ID: 15699522 |
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| Chang HY, Chi JT, Dudoit S, Bondre C, van de Rijn M, Botstein D, Brown PO, Diversity, topographic differentiation, and positional memory in human fibroblasts. Proc Natl Acad Sci U S A99(20):12877-82 2002 |
| PubMed ID: 12297622 |
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| Cristofalo VJ, Allen RG, Pignolo RJ, Martin BG, Beck JC, Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation. Proc Natl Acad Sci U S A95:10614-9 1998 |
| PubMed ID: 9724752 |
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| Allen RG, Keogh BP, Gerhard GS, Pignolo R, Horton J, Cristofalo VJ, Expression and regulation of superoxide dismutase activity in human skin fibroblasts from donors of different ages. J Cell Physiol165:576-87 1995 |
| PubMed ID: 7593237 |
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| Conover CA, Rosenfeld RG, Hintz RL, Insulin-like growth factor II binding and action in human fetal fibroblasts. J Cell Physiol133:560-6 1987 |
| PubMed ID: 2961773 |
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| Clemmons DR, Elgin RG, Han VK, Casella SJ, D'Ercole AJ, Van Wyk JJ, Cultured fibroblast monolayers secrete a protein that alters the cellular binding of somatomedin-C/insulinlike growth factor I. J Clin Invest77:1548-56 1986 |
| PubMed ID: 2422210 |
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| Conover CA, Rosenfeld RG, Hintz RL, Hormonal control of the replication of human fetal fibroblasts: role of somatomedin C/insulin-like growth factor I. J Cell Physiol128:47-54 1986 |
| PubMed ID: 3013906 |
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| Wilhelm, Human fibroblast collagenase: Glycosylat ion and tissue-specific levels of enzyme synthesis. Proc Natl Acad Sci USA83:3756 (1986):47-54 1986 |
| PubMed ID: 3013906 |
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| Van Riper DA, Absher MP, Lenox RH, Muscarinic receptors on intact human fibroblasts. Absence of receptor activity in adult skin cells. J Clin Invest76:882-6 1985 |
| PubMed ID: 2993368 |
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| Jacobs L, Bean CL, Marx JA, Optimal phenotypic expression times for HPRT mutants induced in foreskin-, skin-, and lung-derived human diploid fibroblasts. Environ Mutagen5:717-31 1983 |
| PubMed ID: 6617601 |
| PDL at Senescence |
0 |
| Cumulative PDL at Freeze |
17 |
| Passage Frozen |
6 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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