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AG04148 Fibroblast from Skin, Arm

Description:

GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)

Affected:

No

Sex:

Male

Age:

56 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection GRC
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Arm
Race White
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XY; diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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PDL at Freeze 4.99
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase and Glucose-6-Phosphate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 

Phenotypic Data

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Remarks The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XY; diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Mollinari C, De Dominicis C, Lupacchini L, Sansone L, Caprini D, Casciola CM, Wang Y, Zhao J, Fini M, Russo M, Garaci E, Merlo D, Detection of Pathological Markers of Neurodegenerative Diseases following Microfluidic Direct Conversion of Patient Fibroblasts into Neurons International journal of molecular sciences23: 2021
PubMed ID: 35216271
 
McCoy MJ1, Paul AJ2, Victor MB1, Richner M1,3, Gabel HW4, Gong H5, Yoo AS1, Ahn TH2,6., LONGO: an R package for interactive gene length dependent analysis for neuronal identity Bioinformatics34:422-428 2018
PubMed ID: 29950021
 
Tan KKB1,2, Lim WWM3, Chai C4, Kukumberg M1, Lim KL3,4,5, Goh ELK6,7,8, Yim EKF, Sequential Application of Discrete Topographical Patterns Enhances Derivation of Functional Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells Scientific Reports8:9567 2018
PubMed ID: 29934644
 
Parshad RP, Sanford KK, Price FM, Melnick LK, Nee LE, Schapiro MB, Tarone RE, Robbins JH, Fluorescent light-induced chromatid breaks distinguish Alzheimer disease cells from normal cells in tissue culture. Proc Natl Acad Sci U S A93:5146-50 1996
PubMed ID: 8643543
 
Bedard PA, Golds EE, Cytokine-induced expression of mRNAs for chemotactic factors in human synovial cells and fibroblasts. J Cell Physiol154:433-41 1993
PubMed ID: 8425922
 
Sims NR, Finegan JM, Blass JP, Altered metabolic properties of cultured skin fibroblasts in Alzheimer's disease. Ann Neurol21:451-7 1987
PubMed ID: 3109316
 
Sims NR, Blass JP, Phosphofructokinase activity in fibroblasts from patients with Alzheimer's disease and age- and sex-matched controls. Metab Brain Dis1:83-90 1986
PubMed ID: 2977422
 
Peterson C, Gibson GE, Blass JP, Altered calcium uptake in cultured skin fibroblasts from patients with Alzheimer's disease [letter] N Engl J Med312:1063-5 1985
PubMed ID: 3982463
 
Sims NR, Finegan JM, Blass JP, Altered glucose metabolism in fibroblasts from patients with Alzheimer's disease [letter] N Engl J Med313:638-9 1985
PubMed ID: 4022053
 
Wong, Sodium-independent glutamic acid binding in cultured fibroblasts from patients with Huntington's disease. Neurochem Int6:245 (1984):638-9 1984
PubMed ID: 4022053
 
Schneider EL, Monticone R, Smith J, Braunschweiger K, Roberts T, Skin fibroblast cultures derived from members of the Baltimore longitudinal study: a new resource for studies of cellular aging. Cytogenet Cell Genet31:40-6 1981
PubMed ID: 7307581

External Links

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dbSNP dbSNP ID: 20885

Culture Protocols

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Cumulative PDL at Freeze 12.99
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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