AG02603
Fibroblast from Lung, Lung
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
7.53 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The biopsy was taken post-mortem on 8/4/77. The culture was initiated using explants of minced lung tissue. The cell morphology is fibroblastlike. The karyotype is 46,XX; normal diploid female. A matched skin-derived fibroblast culture from same donor is AG02602A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Zhu B, Fisher E, Li L, Zhong P, Yan Z, Feng J, PTBP2 attenuation facilitates fibroblast to neuron conversion by promoting alternative splicing of neuronal genes Stem cell reports18:2268-2282 2022 |
| PubMed ID: 37832540 |
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| Evans L, Milward K, Attanoos R, Clayton A, Errington R, Tabi Z, Macrophage Plasticity and Function in the Lung Tumour Microenvironment Revealed in 3D Heterotypic Spheroid and Explant Models Biomedicines9:2268-2282 2021 |
| PubMed ID: 33804204 |
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| Rowland TJ, Dumbovic G, Hass EP, Rinn JL, Cech TR, Single-cell imaging reveals unexpected heterogeneity of telomerase reverse transcriptase expression across human cancer cell lines Proceedings of the National Academy of Sciences of the United States of America116:18488-18497 2020 |
| PubMed ID: 31451652 |
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| Foroozandeh P, Aziz AA, Mahmoudi M, Effect of Cell Age on Uptake and Toxicity of Nanoparticles: The Overlooked Factor at the Nanobio Interface ACS applied materials & interfaces116:18488-18497 2019 |
| PubMed ID: 31633323 |
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| Forsyth NR, Evans AP, Shay JW, Wright WE, Developmental differences in the immortalization of lung fibroblasts by telomerase. Aging Cell2(5):235-43 2003 |
| PubMed ID: 14570231 |
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| Boothman DA, Bouvard I, Hughes EN, Identification and characterization of X-ray-induced proteins in human cells. Cancer Res49:2871-8 1989 |
| PubMed ID: 2720648 |
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| McNearney T, Ballard L, Seya T, Atkinson JP, Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. J Clin Invest84:538-45 1989 |
| PubMed ID: 2474570 |
| Cumulative PDL at Freeze |
10.53 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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