AG02262
Fibroblast from Lung, Lung
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
7.88 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy was taken 20 hr post-mortem on 3/18/77. Cause of death was coronary artery disease. The culture was initiated using explants of minced lung tissue. The karyotype is 46,XY; normal diploid male. A matched skin derived fibroblast culture from same donor is AG02261B. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Woods EL, Grigorieva IV, Midgley AC, Brown CVM, Lu YA, Phillips AO, Bowen T, Meran S, Steadman R, CD147 mediates the CD44s-dependent differentiation of myofibroblasts driven by transforming growth factor-ß The Journal of biological chemistry:100987 2021 |
| PubMed ID: 34364871 |
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| Schrank S, McDaid J, Briggs CA, Mustaly-Kalimi S, Brinks D, Houcek A, Singer O, Bottero V, Marr RA, Stutzmann GE, Human-Induced Neurons from Presenilin 1 Mutant Patients Model Aspects of Alzheimer's Disease Pathology International journal of molecular sciences21:100987 2020 |
| PubMed ID: 32033164 |
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| Foroozandeh P, Aziz AA, Mahmoudi M, Effect of Cell Age on Uptake and Toxicity of Nanoparticles: The Overlooked Factor at the Nanobio Interface ACS applied materials & interfaces21:100987 2019 |
| PubMed ID: 31633323 |
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| Midgley AC, Woods E, Jenkins R, Brown C, Khalid U, Chavez R, Hascall V, Steadman R, Phillips AO, Meran S, Hyaluronidase-2 Regulates RhoA Signalling, Myofibroblast Contractility and other Key Pro-fibrotic Myofibroblast Functions The American journal of pathology21:100987 2019 |
| PubMed ID: 32201263 |
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| Bérubé J, Garand C, Lettre G, Lebel M, The non-synonymous polymorphism at position 114 of the WRN protein affects cholesterol efflux in vitro and correlates with cholesterol levels in vivo Experimental gerontology21:100987 2012 |
| PubMed ID: 23523974 |
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| Meran S, Martin J, Luo DD, Steadman R, Phillips A, Interleukin-1ß Induces Hyaluronan and CD44-Dependent Cell Protrusions That Facilitate Fibroblast-Monocyte Binding The American journal of pathology21:100987 2012 |
| PubMed ID: 23583650 |
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| Portnoy J, Pan T, Dinarello CA, Shannon JM, Westcott JY, Zhang L, Mason RJ, Alveolar type II cells inhibit fibroblast proliferation: role of IL-1alpha American journal of physiology Lung cellular and molecular physiology290:L307-16 2005 |
| PubMed ID: 16169897 |
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| Jenkins RH, Thomas GJ, Williams JD, Steadman R, Myofibroblastic differentiation leads to hyaluronan accumulation through reduced hyaluronan turnover. J Biol Chem279(40):41453-60 2004 |
| PubMed ID: 15271981 |
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| Forsyth NR, Evans AP, Shay JW, Wright WE, Developmental differences in the immortalization of lung fibroblasts by telomerase. Aging Cell2(5):235-43 2003 |
| PubMed ID: 14570231 |
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| Pan T, Mason RJ, Westcott JY, Shannon JM, Rat alveolar type II cells inhibit lung fibroblast proliferation in vitro. Am J Respir Cell Mol Biol25(3):353-61 2001 |
| PubMed ID: 11588014 |
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| Shannon JM, Pan T, Nielsen LD, Edeen KE, Mason RJ, Lung fibroblasts improve differentiation of rat type II cells in primary culture. Am J Respir Cell Mol Biol24(3):235-44 2001 |
| PubMed ID: 11245622 |
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| Panos RJ, Rubin JS, Csaky KG, Aaronson SA, Mason RJ, Keratinocyte growth factor and hepatocyte growth factor/scatter factor are heparin-binding growth factors for alveolar type II cells in fibroblast-conditioned medium [published erratum appears in J Clin Invest 1994 Mar;93(3):1347] J Clin Invest92:969-77 1993 |
| PubMed ID: 7688769 |
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| Tong AW, Lee J, Stone MJ, Characterization of two human small cell lung carcinoma-reactive monoclonal antibodies generated by a novel immunization approach. Cancer Res44:4987-92 1984 |
| PubMed ID: 6207911 |
| Cumulative PDL at Freeze |
11.9 |
| Passage Frozen |
3 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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