AG01523
Fibroblast from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Foreskin
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.62 |
| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| INSULIN-LIKE GROWTH FACTOR I AND II |
Yee et al (Cancer Res 48:6691-6696,1988) observed that this normal foreskin fibroblast culture produced high levels of insulin-like growth factor II mRNA. |
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| Remarks |
The culture was initiated on 1/21/76 from explants of minced foreskin removed ante-mortem. The cell morphology is fibroblast-like. The karyotype is 46,XY with 6% of the cells examined showing random chromosome loss/gain and 4% showing 46,XY,del(12)(q12q12)dir ins(12)(p13q11q12). The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Lin CY, Basu K, Ruusala A, Kozlova I, Li YS, Skandalis SS, Heldin CH, Heldin P, Hyaluronan-Induced CD44-iASPP Interaction Affects Fibroblast Migration and Survival Cancers15: 2023 |
| PubMed ID: 36831425 |
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| Mavrogonatou E, Angelopoulou M, Rizou SV, Pratsinis H, Gorgoulis VG, Kletsas D, Activation of the JNKs/ATM-p53 axis is indispensable for the cytoprotection of dermal fibroblasts exposed to UVB radiation Cell death & disease13:647 2022 |
| PubMed ID: 35879280 |
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| Papastathopoulos A, Lougiakis N, Kostakis IK, Marakos P, Pouli N, Pratsinis H, Kletsas D, New bioactive 5-arylcarboximidamidopyrazolo[3,4-c]pyridines: Synthesis, cytotoxic activity, mechanistic investigation and structure-activity relationships European journal of medicinal chemistry218:113387 2021 |
| PubMed ID: 33774342 |
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| Papadopoulou A, Kanioura A, Petrou PS, Argitis P, Kakabakos SE, Kletsas D, Reacquisition of a spindle cell shape does not lead to the restoration of a youthful state in senescent human skin fibroblasts Biogerontology218:113387 2020 |
| PubMed ID: 32533368 |
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| Kanioura A, Petrou P, Kletsas D, Tserepi A, Chatzichristidi M, Gogolides E, Kakabakos S, Three-dimensional (3D) hierarchical oxygen plasma micro/nanostructured polymeric substrates for selective enrichment of cancer cells from mixtures with normal ones Colloids and surfaces B, Biointerfaces218:110675 2019 |
| PubMed ID: 31810566 |
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| Konstantinou D, Mavrogonatou E, Zervou SK, Giannogonas P, Gkelis S, Bioprospecting Sponge-Associated Marine Cyanobacteria to Produce Bioactive Compounds Toxins12:110675 2019 |
| PubMed ID: 31979262 |
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| Yin R, Eger G, Sarri N, Rorsman C, Heldin CH, Lennartsson J, Dual specificity phosphatase (DUSP)-4 is induced by platelet-derived growth factor -BB in an Erk1/2-, STAT3- and p53-dependent manner Biochemical and biophysical research communications519:469-474 2019 |
| PubMed ID: 31526568 |
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| Bourkoula A, Mavrogonatou E, Pavli P, Petrou PS, Douvas AM, Argitis P, Kletsas D, Kakabakos SE., Guided cell adhesion, orientation, morphology and differentiation on silicon substrates photolithographically micropatterned with a cell-repellent cross-linked poly(vinyl alcohol) film Biomedical Materials14:469-474 2018 |
| PubMed ID: 30362459 |
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| Lanemo Myhrinder A, Hellqvist E, Sidorova E, Söderberg A, Baxendale H, Dahle C, Willander K, Tobin G, Bäckman E, Söderberg O, Rosenquist R, Hörkkö S, Rosén A, A new perspective: molecular motifs on oxidized LDL, apoptotic cells, and bacteria are targets for chronic lymphocytic leukemia antibodies Blood111:3838-48 2008 |
| PubMed ID: 18223168 |
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| Dahlström M, Capala J, Lindström P, Wasteson A, Lindström A, Accumulation of boron in human malignant glioma cells in vitro is cell type dependent Journal of neuro-oncology68:199-205 2004 |
| PubMed ID: 15332322 |
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| Demoulin JB, Ericsson J, Kallin A, Rorsman C, Ronnstrand L, Heldin CH, Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins. J Biol ChemEpub ahead of print:199-205 2004 |
| PubMed ID: 15213220 |
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| Stromberg T, Dimberg A, Hammarberg A, Carlson K, Osterborg A, Nilsson K, Jernberg-Wiklund H, Rapamycin sensitizes multiple myeloma cells to apoptosis induced by dexamethasone. Blood103(8):3138-47 2004 |
| PubMed ID: 15070696 |
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| Levina EM, Kharitonova MA, Rovensky YA, Vasiliev JM, Cytoskeletal control of fibroblast length: experiments with linear strips of substrate. J Cell Sci114(Pt 23):4335-41 2001 |
| PubMed ID: 11739665 |
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| Georgii-Hemming P, Stromberg T, Janson ET, Stridsberg M, Wiklund HJ, Nilsson K, The somatostatin analog octreotide inhibits growth of interleukin-6 (IL-6)-dependent and IL-6-independent human multiple myeloma cell lines. Blood93:1724-31 1999 |
| PubMed ID: 10029602 |
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| Wiseman PW, Petersen NO, Image correlation spectroscopy. II. Optimization for ultrasensitive detection of preexisting platelet-derived growth factor-beta receptor oligomers on intact cells. Biophys J76:963-77 1999 |
| PubMed ID: 9916027 |
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| Yam CH, Ng RW, Siu WY, Lau AW, Poon RY, Regulation of cyclin A-Cdk2 by SCF component Skp1 and F-box protein Skp2. Mol Cell Biol19:635-45 1999 |
| PubMed ID: 9858587 |
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| Spets H, Georgii-Hemming P, Siljason J, Nilsson K, Jernberg-Wiklund H, Fas/APO-1 (CD95)-mediated apoptosis is activated by interferon-gamma and interferon- in interleukin-6 (IL-6)-dependent and IL-6-independent multiple myeloma cell lines. Blood92:2914-23 1998 |
| PubMed ID: 9763578 |
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| Schneller M, Vuori K, Ruoslahti E, Alphavbeta3 integrin associates with activated insulin and PDGFbeta receptors and potentiates the biological activity of PDGF. EMBO J16(18):5600-7 1997 |
| PubMed ID: 9312019 |
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| Spets H, Jernberg-Wiklund H, Sambade C, Soderberg O, Nilsson K, The effects on growth and survival of IL-6 can be dissociated in the U-266-1970/U-266-1984 and HL407E/HL407L human multiple myeloma cell lines. Br J Haematol98:126-33 1997 |
| PubMed ID: 9233575 |
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| Poon RYC, Jiang W, Toyoshima H, Hunter T, Cyclin-dependent kinases are inactivated by a combination of p21 and Thr-14/Tyr-15 phosphorylation after UV-induced DNA damage. J Biol Chem271:13283-91 1996 |
| PubMed ID: 8662825 |
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| Eriksson A, Nanberg E, Ronnstrand L, Engstrom U, Hellman U, Rupp E, Carpenter G, Heldin CH, Claesson-Welsh L, Demonstration of functionally different interactions between phospholipase C-gamma and the two types of platelet-derived growth factor receptors. J Biol Chem270:7773-81 1995 |
| PubMed ID: 7535778 |
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| Svitkina TM, Rovensky YA, Bershadsky AD, Vasiliev JM, Transverse pattern of microfilament bundles induced in epitheliocytes by cylindrical substrata. J Cell Sci108 ( Pt 2):735-45 1995 |
| PubMed ID: 7769015 |
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| Karlsson C, Paulsson Y, Age related induction of platelet-derived growth factor A-chain mRNA in normal human fibroblasts. J Cell Physiol158:256-62 1994 |
| PubMed ID: 8106562 |
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| Lassing I, Mellstrom K, Nister M, Comparison of PDGF-AA- and PDGF-BB-induced phosphoinositide formation in human and mouse fibroblasts. Exp Cell Res211:286-95 1994 |
| PubMed ID: 8143775 |
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| Heckman CA, Oravecz KI, Schwab D, Ponten J, Ruffling and locomotion: role in cell resistance to growth factor- induced proliferation. J Cell Physiol154:554-65 1993 |
| PubMed ID: 8436604 |
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| Paulsson Y, Karlsson C, Heldin CH, Westermark B, Density-dependent inhibitory effect of transforming growth factor-beta 1 on human fibroblasts involves the down-regulation of platelet-derived growth factor alpha-receptors. J Cell Physiol157:97-103 1993 |
| PubMed ID: 8408247 |
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| Walz TM, Malm C, Wasteson A, Expression of the transforming growth factor alpha protooncogene in differentiating human promyelocytic leukemia (HL-60) cells. Cancer Res53:191-6 1993 |
| PubMed ID: 7677980 |
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| Loetscher H, Pan YC, Lahm HW, Gentz R, Brockhaus M, Tabuchi H, Lesslauer W, Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor. Cell61:351-9 1990 |
| PubMed ID: 2158862 |
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| Altenburger W, Suter CP, Altenburger J, Partial deletion of the human host range gene in the attenuated vaccinia virus MVA. Arch Virol105:15-27 1989 |
| PubMed ID: 2719552 |
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| Betsholtz C, Svensson V, Rorsman F, Engstrom U, Westermark GT, Wilander E, Johnson K, Westermark P, Islet amyloid polypeptide (IAPP):cDNA cloning and identification of an amyloidogenic region associated with the species-specific occurrence of age-related diabetes mellitus. Exp Cell Res183:484-93 1989 |
| PubMed ID: 2670595 |
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| Bizik J, Lizonova A, Grofova M, Matoska J, Dore JF, Bertrand S, Blasko M, Vaheri A, Clonal variation in the production of tumor-associated alpha 2- macroglobulin in a malignant human melanoma and association with growth stimulation. Cancer Res49:983-90 1989 |
| PubMed ID: 2463878 |
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| Claesson-Welsh L, Hammacher A, Westermark B, Heldin CH, Nister M, Identification and structural analysis of the A type receptor for platelet-derived growth factor. Similarities with the B type receptor. J Biol Chem264:1742-7 1989 |
| PubMed ID: 2536372 |
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| Finch PW, Rubin JS, Miki T, Ron D, Aaronson SA, Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth. Science245:752-5 1989 |
| PubMed ID: 2475908 |
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| Heldin NE, Paulsson Y, Forsberg K, Heldin CH, Westermark B, Induction of cyclic AMP synthesis by forskolin is followed by a reduction in the expression of c-myc messenger RNA and inhibition of 3H- thymidine incorporation in human fibroblasts. J Cell Physiol138:17-23 1989 |
| PubMed ID: 2536035 |
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| Hosang M, Rouge M, Wipf B, Eggimann B, Kaufmann F, Hunziker W, Both homodimeric isoforms of PDGF (AA and BB) have mitogenic and chemotactic activity and stimulate phosphoinositol turnover. J Cell Physiol140:558-64 1989 |
| PubMed ID: 2550476 |
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| Seifert RA, Hart CE, Phillips PE, Forstrom JW, Ross R, Murray MJ, Bowen-Pope DF, Two different subunits associate to create isoform-specific platelet- derived growth factor receptors. J Biol Chem264:8771-8 1989 |
| PubMed ID: 2542288 |
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| Forsberg K, Paulsson Y, Westermark B, Effect on platelet-derived growth factor-induced mitogenesis of double- stranded RNA: evidence for an autocrine growth inhibition mediated by interferon-beta. J Cell Physiol136:266-72 1988 |
| PubMed ID: 2842345 |
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| Heldin CH, Backstrom G, Ostman A, Hammacher A, Ronnstrand L, Rubin K, Nister M, Westermark B, Binding of different dimeric forms of PDGF to human fibroblasts: evidence for two separate receptor types. EMBO J7:1387-93 1988 |
| PubMed ID: 2842148 |
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| Hosang M, Recombinant interferon-gamma inhibits the mitogenic effect of platelet- derived growth factor at a level distal to the growth factor receptor. J Cell Physiol134:396-404 1988 |
| PubMed ID: 2832421 |
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| Mellstrom K, Heldin CH, Westermark B, Induction of circular membrane ruffling on human fibroblasts by platelet-derived growth factor. Exp Cell Res177:347-59 1988 |
| PubMed ID: 3391248 |
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| Nagasawa H, Burke MJ, Little FF, McCone EF, Chan GL, Little JB, Multiple abnormalities in the ultraviolet light response of cultured fibroblasts derived from patients with the basal cell nevus syndrome. Teratog Carcinog Mutagen8:25-33 1988 |
| PubMed ID: 2897722 |
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| Yee D, Cullen KJ, Paik S, Perdue JF, Hampton B, Schwartz A, Lippman ME, Rosen N, Insulin-like growth factor II mRNA expression in human breast cancer. Cancer Res48:6691-6 1988 |
| PubMed ID: 3180080 |
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| Bronzert DA, Pantazis P, Antoniades HN, Kasid A, Davidson N, Dickson RB, Lippman ME, Synthesis and secretion of platelet-derived growth factor by human breast cancer cell lines. Proc Natl Acad Sci U S A84:5763-7 1987 |
| PubMed ID: 3039506 |
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| Connolly DT, Stoddard BL, Harakas NK, Feder J, Human fibroblast-derived growth factor is a mitogen and chemoattractant for endothelial cells. Biochem Biophys Res Commun144:705-12 1987 |
| PubMed ID: 3579937 |
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| Paulsson Y, Bywater M, Heldin CH, Westermark B, Effects of epidermal growth factor and platelet-derived growth factor on c-fos and c-myc mRNA levels in normal human fibroblasts. Exp Cell Res171:186-94 1987 |
| PubMed ID: 3497813 |
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| Betsholtz C, Johnsson A, Heldin CH, Westermark B, Efficient reversion of simian sarcoma virus-transformation and inhibition of growth factor-induced mitogenesis by suramin. Proc Natl Acad Sci U S A83:6440-4 1986 |
| PubMed ID: 3018732 |
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| Betsholtz C, Johnsson A, Heldin CH, Westermark B, Lind P, Urdea MS, Eddy R, Shows TB, Philpott K, Mellor AL, et al, cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. Nature320:695-9 1986 |
| PubMed ID: 3754619 |
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| Clemmons DR, Elgin RG, Han VK, Casella SJ, D'Ercole AJ, Van Wyk JJ, Cultured fibroblast monolayers secrete a protein that alters the cellular binding of somatomedin-C/insulinlike growth factor I. J Clin Invest77:1548-56 1986 |
| PubMed ID: 2422210 |
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| Glenney J, Two related but distinct forms of the Mr 36,000 tyrosine kinase substrate (calpactin) that interact with phospholipid and actin in a Ca2+-dependent manner. Proc Natl Acad Sci U S A83:4258-62 1986 |
| PubMed ID: 3012561 |
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| Isacke CM, Sauvage CA, Hyman R, Lesley J, Schulte R, Trowbridge IS, Identification and characterization of the human Pgp-1 glycoprotein. Immunogenetics23:326-32 1986 |
| PubMed ID: 3519447 |
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| Isacke CM, Trowbridge IS, Hunter T, Modulation of p36 phosphorylation in human cells: studies using anti- p36 monoclonal antibodies. Mol Cell Biol6:2745-51 1986 |
| PubMed ID: 2946941 |
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| Paulsson Y, Bywater M, Pfeifer-Ohlsson S, Ohlsson R, Nilsson S, Heldin CH, Westermark B, Betsholtz C, Growth factors induce early pre-replicative changes in senescent human fibroblasts. EMBO J5:2157-62 1986 |
| PubMed ID: 3023058 |
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| Westermark B, Johnsson A, Paulsson Y, Betsholtz C, Heldin CH, Herlyn M, Rodeck U, Koprowski H, Human melanoma cell lines of primary and metastatic origin express the genes encoding the chains of platelet-derived growth factor (PDGF) and produce a PDGF-like growth factor. Proc Natl Acad Sci U S A83:7197-200 1986 |
| PubMed ID: 3020539 |
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| Betsholtz C, Bywater M, Westermark B, Burk RR, Heldin CH, Expression of the c-sis gene and secretion of a platelet-derived growth factor-like protein by simian virus 40-transformed BHK cells. Biochem Biophys Res Commun130:753-60 1985 |
| PubMed ID: 2992485 |
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| Johnsson A, Betsholtz C, Heldin CH, Westermark B, Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus. Nature317:438-40 1985 |
| PubMed ID: 2995828 |
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| Johnsson A, Betsholtz C, von der Helm K, Heldin CH, Westermark B, Platelet-derived growth factor agonist activity of a secreted form of the v-sis oncogene product. Proc Natl Acad Sci U S A82:1721-5 1985 |
| PubMed ID: 2984666 |
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| Karlsson R, Lindberg U, Changes in the organization of actin and myosin in non-muscle cells induced by N-ethylmaleimide. Exp Cell Res157:95-115 1985 |
| PubMed ID: 3882439 |
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| Nilsson J, Sjolund M, Palmberg L, Thyberg J, Heldin CH, Arterial smooth muscle cells in primary culture produce a platelet- derived growth factor-like protein. Proc Natl Acad Sci U S A82:4418-22 1985 |
| PubMed ID: 2989820 |
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| Westermark B, Heldin CH, Similar action of platelet-derived growth factor and epidermal growth factor in the prereplicative phase of human fibroblasts suggests a common intracellular pathway. J Cell Physiol124:43-8 1985 |
| PubMed ID: 3876339 |
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| Betsholtz C, Westermark B, Growth factor-induced proliferation of human fibroblasts in serum-free culture depends on cell density and extracellular calcium concentration. J Cell Physiol118:203-10 1984 |
| PubMed ID: 6607257 |
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| Betsholtz C, Westermark B, Ek B, Heldin CH, Coexpression of a PDGF-like growth factor and PDGF receptors in a human osteosarcoma cell line: implications for autocrine receptor activation. Cell39:447-57 1984 |
| PubMed ID: 6096004 |
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| Nister M, Heldin CH, Wasteson A, Westermark B, A glioma-derived analog to platelet-derived growth factor: demonstration of receptor competing activity and immunological crossreactivity. Proc Natl Acad Sci U S A81:926-30 1984 |
| PubMed ID: 6322178 |
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| Nilsson J, Thyberg J, Heldin CH, Westermark B, Wasteson A, Surface binding and internalization of platelet-derived growth factor in human fibroblasts. Proc Natl Acad Sci U S A80:5592-6 1983 |
| PubMed ID: 6351063 |
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| Cooper JA, Bowen-Pope DF, Raines E, Ross R, Hunter T, Similar effects of platelet-derived growth factor and epidermal growth factor on the phosphorylation of tyrosine in cellular proteins. Cell31:263-73 1982 |
| PubMed ID: 6186382 |
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| Ek B, Westermark B, Wasteson A, Heldin CH, Stimulation of tyrosine-specific phosphorylation by platelet-derived growth factor. Nature295:419-20 1982 |
| PubMed ID: 6173766 |
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| Heldin CH, Westermark B, Wasteson A, Specific receptors for platelet-derived growth factor on cells derived from connective tissue and glia. Proc Natl Acad Sci U S A78:3664-8 1981 |
| PubMed ID: 6943569 |
| Passage Frozen |
7 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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