AG01522
Fibroblast from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Foreskin
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
16 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The culture was initiated on 1/21/76 from explants of minced foreskin removed ante-mortem 1 day earlier. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cahoon P, Giacometti V, Casey F, Russell E, McGarry CK, Prise KM, McMahon SJ, Investigating spatial fractionation and radiation induced bystander effects: a mathematical modelling approach Physics in medicine and biology: 2021 |
| PubMed ID: 34666318 |
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| Domogauer JD, de Toledo SM, Howell RW, Azzam EI, Acquired radioresistance in cancer associated fibroblasts is concomitant with enhanced antioxidant potential and DNA repair capacity Cell communication and signaling : CCS19:30 2020 |
| PubMed ID: 33637118 |
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| Kalen AL, Wagner BA, Sarsour EH, Kumar MG, Reedy JL, Buettner GR, Barua NC, Goswami PC, Hydrogen Peroxide Mediates Artemisinin-Derived C-16 Carba-Dimer-Induced Toxicity of Human Cancer Cells Antioxidants (Basel, Switzerland)9:30 2019 |
| PubMed ID: 31991904 |
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| Muoio D1, Berardinelli F1, Leone S1, Coluzzi E1, di Masi A1, Doria F2, Freccero M2, Sgura A1, Folini M3, Antoccia A1., Naphthalene diimide-derivatives G-quadruplex ligands induce cell proliferation inhibition, mild telomeric dysfunction and cell cycle perturbation in U251MG glioma cells FEBS Journal9:30 2018 |
| PubMed ID: 30095224 |
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| Wojtaszek JL, Chatterjee N, Najeeb J, Ramos A, Lee M, Bian K, Xue JY, Fenton BA, Park H, Li D, Hemann MT, Hong J, Walker GC, Zhou P, A Small Molecule Targeting Mutagenic Translesion Synthesis Improves Chemotherapy Cell178:152-159.e11 2018 |
| PubMed ID: 31178121 |
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| Kalen AL, Ahmad IM, Abdalla MY, O'Malley YQ, Goswami PC, Sarsour EH, MnSOD and Cyclin B1 Coordinate a Mito-Checkpoint during Cell Cycle Response to Oxidative Stress Antioxidants (Basel, Switzerland)6:152-159.e11 2017 |
| PubMed ID: 29149089 |
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| Sharma NK, Lebedeva M, Thomas T, Kovalenko OA, Stumpf JD, Shadel GS, Santos JH, Intrinsic mitochondrial DNA repair defects in Ataxia Telangiectasia DNA Repair (AMST)13:22-31 2014 |
| PubMed ID: 24342190 |
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| Eckers JC, Kalen AL, Xiao W, Sarsour EH, Goswami PC., Selenoprotein P inhibits radiation-induced late reactive oxygen species accumulation and normal cell injury. Int J Radiat Oncol Biol Phys.87(3):619-25 2013 |
| PubMed ID: 24074935 |
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| Liu C, Kawata T, Furusawa Y, Zhou G, Inoue K, Fukada J, Kota R, George K, Cucinotta F, Okayasu R, Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET) Mutat Res756(1-2):101-7 2013 |
| PubMed ID: 23688614 |
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| Liu C, Kawata T, Zhou G, Furusawa Y, Kota R, Kumabe A, Sutani S, Fukada J, Mishima M, Shigematsu N, George K, Cucinotta F, Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from the kinetics and fidelity of chromosome rejoining in normal human fibroblasts J Radiat Res54(6):989-97 2013 |
| PubMed ID: 23674607 |
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| Sakashita T, Hamada N, Kawaguchi I, Ouchi NB, Hara T, Kobayashi Y, Saito K, A framework for analysis of abortive colony size distributions using a model of branching processes in irradiated normal human fibroblasts PLoS One8(7):e70291 2013 |
| PubMed ID: 23894635 |
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| Autsavapromporn N, De Toledo SM, Jay-Gerin JP, Harris AL, Azzam EI, Human cell responses to ionizing radiation are differentially affected by the expressed connexins Journal of radiation research54:251-9 2012 |
| PubMed ID: 23139176 |
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| Butterworth KT, McGarry CK, Trainor C, McMahon SJ, O'Sullivan JM, Schettino G, Hounsell AR, Prise KM, Dose, dose-rate and field size effects on cell survival following exposure to non-uniform radiation fields Physics in medicine and biology57:3197-206 2012 |
| PubMed ID: 22546687 |
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| Kumar MG, Patel NM, Nicholson AM, Kalen AL, Sarsour EH, Goswami PC, Reactive oxygen species mediate microRNA-302 regulation of AT-Rich Interacting Domain 4a and C-C motif Ligand 5 expression during transitions between quiescence and proliferation Free radical biology & medicine57:3197-206 2012 |
| PubMed ID: 22732186 |
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| Wakatsuki M, Magpayo N, Kawamura H, Held KD, Differential Bystander Signaling Between Radioresistant Chondrosarcoma Cells and Fibroblasts After X-Ray, Proton, Iron Ion and Carbon Ion Exposures International journal of radiation oncology, biology, physics57:3197-206 2012 |
| PubMed ID: 22537542 |
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| Wiese C, Rudolph JH, Jakob B, Fink D, Tobias F, Blattner C, Taucher-Scholz G, PCNA-dependent accumulation of CDKN1A into nuclear foci after ionizing irradiation DNA repair57:3197-206 2011 |
| PubMed ID: 22456500 |
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| Wilson PF, Nagasawa H, Warner CL, Fitzek MM, Little JB, Bedford JS, Radiation Sensitivity of Primary Fibroblasts from Hereditary Retinoblastoma Family Members and Some Apparently Normal Controls: Colony Formation Ability during Continuous Low-Dose-Rate Gamma Irradiation Radiation research169:483-94 2008 |
| PubMed ID: 18439048 |
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| Pinto M, Howell RW, Concomitant quantification of targeted drug delivery and biological response in individual cells BioTechniques43:64, 66-71 2007 |
| PubMed ID: 17695254 |
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| Venkatachalam P, de Toledo SM, Pandey BN, Tephly LA, Carter AB, Little JB, Spitz DR, Azzam EI, Regulation of normal cell cycle progression by flavin-containing oxidases Oncogene27:20-31 2007 |
| PubMed ID: 17637756 |
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| Campa A, Ballarini F, Belli M, Cherubini R, Dini V, Esposito G, Friedland W, Gerardi S, Molinelli S, Ottolenghi A, Paretzke H, Simone G, Tabocchini MA, DNA DSB induced in human cells by charged particles and gamma rays: experimental results and theoretical approaches International journal of radiation biology81:841-54 2006 |
| PubMed ID: 16484153 |
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| Pinto M, Azzam EI, Howell RW, Bystander responses in three-dimensional cultures containing radiolabelled and unlabelled human cells Radiation protection dosimetry122:252-5 2006 |
| PubMed ID: 17185313 |
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| Ben-Omran TI, Cerosaletti K, Concannon P, Weitzman S, Nezarati MM, A patient with mutations in DNA Ligase IV: Clinical features and overlap with Nijmegen breakage syndrome. Am J Med Genet A137(3):283-7 2005 |
| PubMed ID: 16088910 |
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| Sarsour EH, Agarwal M, Pandita TK, Oberley LW, Goswami PC, Manganese superoxide dismutase protects the proliferative capacity of confluent normal human fibroblasts. J Biol Chem280(18):18033-41 2005 |
| PubMed ID: 15743756 |
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| Yang H, Asaad N, Held KD, Medium-mediated intercellular communication is involved in bystander responses of X-ray-irradiated normal human fibroblasts Oncogene24:2096-103 2005 |
| PubMed ID: 15688009 |
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| Fournier C, Wiese C, Taucher-Scholz G, Accumulation of the cell cycle regulators TP53 and CDKN1A (p21) in human fibroblasts after exposure to low- and high-LET radiation. Radiat Res161(6):675-84 2004 |
| PubMed ID: 15161352 |
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| Jensen R, Glazer PM, Cell-interdependent cisplatin killing by Ku/DNA-dependent protein kinase signaling transduced through gap junctions. Proc Natl Acad Sci U S A101(16):6134-9 2004 |
| PubMed ID: 15069205 |
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| Nasonova E, Füssel K, Berger S, Gudowska-Nowak E, Ritter S, Cell cycle arrest and aberration yield in normal human fibroblasts I Effects of X-rays and 195 MeV u(-1) C ions International journal of radiation biology80:621-34 2004 |
| PubMed ID: 15586882 |
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| Neti PV, de Toledo SM, Perumal V, Azzam EI, Howell RW, A multi-port low-fluence alpha-particle irradiator: fabrication, testing and benchmark radiobiological studies Radiation research161:732-8 2004 |
| PubMed ID: 15161346 |
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| Jakob B, Scholz M, Taucher-Scholz G, Biological imaging of heavy charged-particle tracks Radiation research159:676-84 2003 |
| PubMed ID: 12710880 |
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| Shao C, Furusawa Y, Aoki M, Ando K, Role of gap junctional intercellular communication in radiation-induced bystander effects in human fibroblasts Radiation research160:318-23 2003 |
| PubMed ID: 12926990 |
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| Shao C, Furusawa Y, Kobayashi Y, Funayama T, Wada S, Bystander effect induced by counted high-LET particles in confluent human fibroblasts: a mechanistic study. FASEB J17(11):1422-7 2003 |
| PubMed ID: 12890695 |
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| Azzam EI, De Toledo SM, Spitz DR, Little JB, Oxidative metabolism modulates signal transduction and micronucleus formation in
bystander cells from alpha-particle-irradiated normal human fibroblast cultures. Cancer Res62(19):5436-42 2002 |
| PubMed ID: 12359750 |
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| Barton GM, Medzhitov R, Retroviral delivery of small interfering RNA into primary cells. Proc Natl Acad Sci U S A99(23):14943-5 2002 |
| PubMed ID: 12417750 |
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| Jakob B, Scholz M, Taucher-Scholz G, Characterization of CDKN1A (p21) binding to sites of heavy-ion-induced damage: colocalization with proteins involved in DNA repair International journal of radiation biology78:75-88 2002 |
| PubMed ID: 11779358 |
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| Pinto M, Prise KM, Michael BD, Quantification of radiation induced DNA double-strand breaks in human fibroblasts by PFGE: testing the applicability of random breakage models. Int J Radiat Biol78(5):375-88 2002 |
| PubMed ID: 12020428 |
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| Pinto M, Prise KM, Michael BD, Double strand break rejoining after irradiation of human fibroblasts with X rays or alpha particles: PFGE studies and numerical models. Radiat Prot Dosimetry99(1-4):133-6 2002 |
| PubMed ID: 12194265 |
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| Azzam EI, de Toledo SM, Little JB, Direct evidence for the participation of gap junction-mediated intercellular communication in the transmission of damage signals from alpha -particle irradiated to nonirradiated cells. Proc Natl Acad Sci U S A98(2):473-8 2001 |
| PubMed ID: 11149936 |
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| Fournier C, Scholz M, Weyrather WK, Rodemann HP, Kraft G, Changes of fibrosis-related parameters after high- and low-LET irradiation of fibroblasts. Int J Radiat Biol77(6):713-722 2001 |
| PubMed ID: 11403711 |
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| Prise KM, Pinto M, Newman HC, Michael BD, A review of studies of ionizing radiation-induced double-strand break clustering. Radiat Res156(5 Pt 2):572-6 2001 |
| PubMed ID: 11604074 |
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| Broome EJ, Brown DL, Mitchel RE, Adaptation of human fibroblasts to radiation alters biases in DNA repair at the chromosomal level. Int J Radiat Biol75(6):681-90 1999 |
| PubMed ID: 10404997 |
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| Diaz-Zagoya JC, Asenjo-Barron JC, Cardenas-Vazquez R, Martinez F, Juarez-Oropeza MA, Comparative toxicity of high doses of vastatins currently used by clinicians, in CD-1 male mice fed with a hypercholesterolemic diet. Life Sci65(9):947-56 1999 |
| PubMed ID: 10465354 |
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| Smilenov LB, Dhar S, Pandita TK, Altered telomere nuclear matrix interactions and nucleosomal periodicity in ataxia telangiectasia cells before and after ionizing radiation treatment. Mol Cell Biol19:6963-71 1999 |
| PubMed ID: 10490633 |
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| Dahlberg, Response of dermal fibroblast cultures from patients eith unusually severe responses to radiotherapy and from ataxia telangiectasia heterozygotes to fractionated radiation. Clin Cancer Res1:785 (1995):6963-71 1995 |
| PubMed ID: 10490633 |
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| Saad AH, Kuo SS, Koong AC, Hahn GM, Giaccia AJ, Modulation of potassium channels by protein tyrosine kinase inhibitors. J Cell Physiol161:142-8 1994 |
| PubMed ID: 7929599 |
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| Goodwin E, Meyne J, Strand-specific FISH reveals orientation of chromosome 18 alphoid DNA. Cytogenet Cell Genet63:126-7 1993 |
| PubMed ID: 8467711 |
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| Taylor YC, Parsian AJ, Duncan PG, Differential post-irradiation caffeine response in normal diploid versus SV40-transformed human fibroblasts: potential role of nuclear organization and protein-composition. Int J Radiat Biol64(1):57-70 1993 |
| PubMed ID: 8102171 |
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| Kastan MB, Zhan Q, el-Deiry WS, Carrier F, Jacks T, Walsh WV, Plunkett BS, Vogelstein B, Fornace AJ Jr, A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. Cell71:587-97 1992 |
| PubMed ID: 1423616 |
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| Nagasawa H, Little JB, Tsang NM, Saunders E, Tesmer J, Strniste GF, Effect of dose rate on the survival of irradiated human skin fibroblasts. Radiat Res132:375-9 1992 |
| PubMed ID: 1475361 |
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| Taylor YC, Duncan PG, Zhang X, Wright WD, Differences in the DNA supercoiling response of irradiated cell lines from ataxia-telangiectasia versus unaffected individuals. Int J Radiat Biol59:359-71 1991 |
| PubMed ID: 1671687 |
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| Taylor YC, Zhang XF, Parsian AJ, Duncan PG, Image analysis-based measurement of DNA supercoiling changes in transformed and nontransformed human cell lines. Exp Cell Res197:222-8 1991 |
| PubMed ID: 1659987 |
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| Fornace AJ Jr, Papathanasiou MA, Hollander MC, Yarosh DB, Expression of the O6-methylguanine-DNA methyltransferase gene MGMT in MER+ and MER- human tumor cells. Cancer Res50:7908-11 1990 |
| PubMed ID: 2253230 |
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| Sherman ML, Datta R, Hallahan DE, Weichselbaum RR, Kufe DW, Ionizing radiation regulates expression of the c-jun protooncogene. Proc Natl Acad Sci U S A87:5663-6 1990 |
| PubMed ID: 2116003 |
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| Fornace AJ Jr, Alamo I Jr, Hollander MC, Lamoreaux E, Ubiquitin mRNA is a major stress-induced transcript in mammalian cells. Nucleic Acids Res17:1215-30 1989 |
| PubMed ID: 2537950 |
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| Konefal JB, Taylor YC, The effects of altered fractionation schedules on the survival of human cell lines differing in their proliferative activity and repair capacity. Int J Radiat Oncol Biol Phys17:1007-13 1989 |
| PubMed ID: 2808033 |
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| Little JB, Dahlberg WK, Sensitization of noncycling human diploid cells to X-irradiation by pretreatment with 9-beta-D-arabinofuranosyladenine. Cancer Res49:838-42 1989 |
| PubMed ID: 2912556 |
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| Little JB, Nichols WW, Troilo P, Nagasawa H, Strong LC, Radiation sensitivity of cell strains from families with genetic disorders predisposing to radiation-induced cancer. Cancer Res49:4705-14 1989 |
| PubMed ID: 2758405 |
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| Nagasawa H, Burke MJ, Little FF, McCone EF, Chan GL, Little JB, Multiple abnormalities in the ultraviolet light response of cultured fibroblasts derived from patients with the basal cell nevus syndrome. Teratog Carcinog Mutagen8:25-33 1988 |
| PubMed ID: 2897722 |
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| Nagasawa H, Little JB, Radiosensitivities of ten apparently normal human diploid fibroblast strains to cell killing, G2-phase chromosomal aberrations, and cell cycle delay. Cancer Res48:4535-8 1988 |
| PubMed ID: 3396006 |
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| Heartlein MW, Tsuji H, Latt SA, 5-Bromodeoxyuridine-dependent increase in sister chromatid exchange formation in Bloom's syndrome is associated with reduction in topoisomerase II activity. Exp Cell Res169:245-54 1987 |
| PubMed ID: 3028845 |
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| Nagasawa H, Kraemer KH, Shiloh Y, Little JB, Detection of ataxia telangiectasia heterozygous cell lines by postirradiation cumulative labeling index: measurements with coded samples. Cancer Res47:398-402 1987 |
| PubMed ID: 3791230 |
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| Zamansky GB, Chou IN, Environmental wavelengths of ultraviolet light induce cytoskeletal damage. J Invest Dermatol89:603-6 1987 |
| PubMed ID: 3680985 |
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| Fornace AJ Jr, Kinsella TJ, Dobson PP, Mitchell JB, Repair of ionizing radiation DNA base damage in ataxia-telangiectasia cells. Cancer Res46:1703-6 1986 |
| PubMed ID: 3004702 |
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| Geard CR, Georgsson MA, Glutathione levels and cytotoxicity of a thiol activated alkylating agent in human and mouse cells. Int J Radiat Oncol Biol Phys12:1179-82 1986 |
| PubMed ID: 3744937 |
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| Hards RG, Benkovic SJ, Van Keuren ML, Graw SL, Drabkin HA, Patterson D, Assignment of a third purine biosynthetic gene (glycinamide ribonucleotide transformylase) to human chromosome 21. Am J Hum Genet39:179-85 1986 |
| PubMed ID: 3529945 |
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| Hards RG, Patterson D, Variation of glycinamide ribonucleotide synthetase levels during in vitro aging of human fibroblasts: implications for gene dosage studies. Mech Ageing Dev36:65-70 1986 |
| PubMed ID: 3762234 |
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| Kano Y, Little JB, Site-specific chromosomal rearrangements induced in human diploid cells by x-irradiation. Cytogenet Cell Genet41:22-9 1986 |
| PubMed ID: 3943368 |
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| Nove J, Little JB, Mayer PJ, Troilo P, Nichols WW, Hypersensitivity of cells from a new chromosomal-breakage syndrome to DNA-damaging agents. Mutat Res163:255-62 1986 |
| PubMed ID: 3785261 |
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| Sheela S, Kennedy AR, Radiation-induced anchorage-independent growth and collagenase production in diploid human fibroblasts. Carcinogenesis7:201-5 1986 |
| PubMed ID: 3004774 |
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| Van Keuren ML, Watkins PC, Drabkin HA, Jabs EW, Gusella JF, Patterson D, Regional localization of DNA sequences on chromosome 21 using somatic cell hybrids. Am J Hum Genet38:793-804 1986 |
| PubMed ID: 3014865 |
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| Zamansky GB, Recovery from UV-induced potentially lethal damage in systemic lupus erythematosus skin fibroblasts. Int J Radiat Biol Relat Stud Phys Chem Med50:305-12 1986 |
| PubMed ID: 3488287 |
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| Zamansky GB, Varying sensitivity of human skin fibroblasts to polychromatic ultraviolet light. Mutat Res160:55-60 1986 |
| PubMed ID: 3951456 |
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| Zeman EM, Brown JM, Lemmon MJ, Hirst VK, Lee WW, SR-4233: a new bioreductive agent with high selective toxicity for hypoxic mammalian cells. Int J Radiat Oncol Biol Phys12:1239-42 1986 |
| PubMed ID: 3744945 |
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| Cheng YS, Becker-Manley MF, Chow TP, Horan DC, Affinity purification of an interferon-induced human guanylate-binding protein and its characterization. J Biol Chem260:15834-9 1985 |
| PubMed ID: 3934165 |
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| Cornforth MN, Bedford JS, On the nature of a defect in cells from individuals with ataxia- telangiectasia. Science227:1589-91 1985 |
| PubMed ID: 3975628 |
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| Kano Y, Little JB, Mechanisms of human cell neoplastic transformation: X-ray-induced abnormal clone formation in long-term cultures of human diploid fibroblasts. Cancer Res45:2550-5 1985 |
| PubMed ID: 3986793 |
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| Kano Y, Little JB, Mechanisms of human cell neoplastic transformation: relationship of specific abnormal clone formation to prolonged lifespan in X-irradiated human diploid fibroblasts. Int J Cancer36:407-13 1985 |
| PubMed ID: 4030141 |
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| Little JB, Nagasawa H, Weichselbaum RR, Lovett DR, Schmit A, Kochenburger RJ, Blumberg PM, Differing patterns of cytotoxicity of the phorbol ester 12-O- tetradecanoylphorbol 13-acetate in various human cell strains. Carcinogenesis6:1703-8 1985 |
| PubMed ID: 3864565 |
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| Nagasawa H, Latt SA, Lalande ME, Little JB, Effects of X-irradiation on cell-cycle progression, induction of chromosomal aberrations and cell killing in ataxia telangiectasia (AT) fibroblasts. Mutat Res148:71-82 1985 |
| PubMed ID: 3969079 |
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| Schwenn MR, Weichselbaum RR, Little JB, Investigation of the cytotoxic effects of DNA damaging agents on neurofibromatosis cells. Mutat Res142:55-8 1985 |
| PubMed ID: 3919286 |
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| Weichselbaum RR, Tomkinson K, Little JB, Repair of potentially lethal X-ray damage in fibroblasts derived from patients with hereditary and D-deletion retinoblastoma. Int J Radiat Biol Relat Stud Phys Chem Med47:445-56 1985 |
| PubMed ID: 3872857 |
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| Zamansky GB, Minka DF, Deal CL, Hendricks K, The in vitro photosensitivity of systemic lupus erythematosus skin fibroblasts. J Immunol134:1571-6 1985 |
| PubMed ID: 3968426 |
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| Grafstrom RC, Fornace A Jr, Harris CC, Repair of DNA damage caused by formaldehyde in human cells. Cancer Res44:4323-7 1984 |
| PubMed ID: 6467194 |
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| Kano Y, Little JB, Persistence of X-ray-induced chromosomal rearrangements in long-term cultures of human diploid fibroblasts. Cancer Res44:3706-11 1984 |
| PubMed ID: 6744287 |
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| Kennedy AR, Radner BS, Nagasawa H, Protease inhibitors reduce the frequency of spontaneous chromosome abnormalities in cells from patients with Bloom syndrome. Proc Natl Acad Sci U S A81:1827-30 1984 |
| PubMed ID: 6584916 |
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| Kufe DW, Weichselbaum R, Egan EM, Dahlberg W, Fram RJ, Lethal effects of 1-beta-D-arabinofuranosylcytosine incorporation into deoxyribonucleic acid during ultraviolet repair. Mol Pharmacol25:322-6 1984 |
| PubMed ID: 6700578 |
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| LeMotte PK, Little JB, DNA damage induced in human diploid cells by decay of incorporated radionuclides. Cancer Res44:1337-42 1984 |
| PubMed ID: 6704953 |
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| Chan GL, Little JB, Cultured diploid fibroblasts from patients with the nevoid basal cell carcinoma syndrome are hypersensitive to killing by ionizing radiation. Am J Pathol111:50-5 1983 |
| PubMed ID: 6837723 |
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| Cornforth MN, Bedford JS, High-resolution measurement of breaks in prematurely condensed chromosomes by differential staining. Chromosoma88:315-8 1983 |
| PubMed ID: 6653204 |
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| Cornforth MN, Bedford JS, X-ray--induced breakage and rejoining of human interphase chromosomes. Science222:1141-3 1983 |
| PubMed ID: 6648528 |
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| Grosovsky AJ, Little JB, Mutagenesis and lethality following S phase irradiation of xeroderma pigmentosum and normal human diploid fibroblasts with ultraviolet light. Carcinogenesis4:1389-93 1983 |
| PubMed ID: 6640842 |
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| Grosovsky AJ, Little JB, Effect of growth rate on phenotypic expression of 6-thioguanine resistance in human diploid fibroblasts. Mutat Res110:163-70 1983 |
| PubMed ID: 6865995 |
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| Grosovsky AJ, Little JB, Influence of confluent holding time on UV light mutagenesis in human diploid fibroblasts. Mutat Res110:401-12 1983 |
| PubMed ID: 6877263 |
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| LeMotte PK, Little JB, A comparison of the lethal effects of intracellular radionuclides in human and rodent cells. Radiat Res95:359-69 1983 |
| PubMed ID: 6611854 |
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| Li FP, Little JB, Bech-Hansen NT, Paterson MC, Arlett C, Garnick MB, Mayer RJ, Acute leukemia after radiotherapy in a patient with Turcot's syndrome. Impaired colony formation in skin fibroblast cultures after irradiation. Am J Med74:343-8 1983 |
| PubMed ID: 6572034 |
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| Nagasawa H, Little JB, Suppression of cytotoxic effect of mitomycin-C by superoxide dismutase in Fanconi's anemia and dyskeratosis congenita fibroblasts. Carcinogenesis4:795-9 1983 |
| PubMed ID: 6409437 |
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| Nagasawa H, Little JB, Comparison of kinetics of X-ray-induced cell killing in normal, ataxia telangiectasia and hereditary retinoblastoma fibroblasts. Mutat Res109:297-308 1983 |
| PubMed ID: 6843573 |
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| Zamansky GB, Arundel C, Nagasawa H, Little JB, Adaptation of human diploid fibroblasts in vitro to serum from different sources. J Cell Sci61:289-97 1983 |
| PubMed ID: 6885940 |
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| Barfknecht TR, Little JB, Abnormal sensitivity of skin fibroblasts from familial polyposis patients to DNA alkylating agents. Cancer Res42:1249-54 1982 |
| PubMed ID: 6800649 |
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| Barfknecht TR, Little JB, Survival of hereditary retinoblastoma human skin fibroblasts after treatment with DNA-damaging chemicals. Mutat Res105:189-94 1982 |
| PubMed ID: 6815522 |
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| Barfknecht TR, Little JB, Hypersensitivity of ataxia telangiectasia skin fibroblasts to DNA alkylating agents. Mutat Res94:369-82 1982 |
| PubMed ID: 6810166 |
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| Fornace AJ Jr, Detection of DNA single-strand breaks produced during the repair of damage by DNA-protein cross-linking agents. Cancer Res42:145-9 1982 |
| PubMed ID: 7198506 |
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| Fornace AJ Jr, Measurement of M. luteus endonuclease-sensitive lesions by alkaline elution. Mutat Res94:263-76 1982 |
| PubMed ID: 7110177 |
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| Fornace AJ Jr, Seres DS, Repair of trans-Pt(II) diamminedichloride DNA-protein crosslinks in normal and excision-deficient human cells. Mutat Res94:277-84 1982 |
| PubMed ID: 7202118 |
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| Kinsella TJ, Little JB, Nove J, Weichselbaum RR, Li FP, Meyer RJ, Marchetto DJ, Patterson WB, Heterogeneous response to X-ray and ultraviolet light irradiations of cultured skin fibroblasts in two families with Gardner's Syndrome. J Natl Cancer Inst68:697-701 1982 |
| PubMed ID: 6951083 |
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| Kinsella TJ, Mitchell JB, McPherson S, Russo A, Tietze F, In vitro X-ray sensitivity in ataxia telangiectasis homozygote and heterozygote skin fibroblasts under oxic and hypoxic conditions. Cancer Res42:3950-6 1982 |
| PubMed ID: 7104995 |
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| LeMotte, The relative toxicity of intracellular radioisotopes in human and rodent cells. Radiat Res91:302 (1982):3950-6 1982 |
| PubMed ID: 7104995 |
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| Seres DS, Fornace AJ Jr, Normal response of fanconi's anemia cells to high concentrations of O2 as determined by alkaline elution. Biochim Biophys Acta698:237-42 1982 |
| PubMed ID: 7138867 |
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| Zamansky GB, Little JB, Survival of 60Co-irradiated herpes simplex virus in 15 human diploid fibroblast cell strains. Mutat Res94:245-55 1982 |
| PubMed ID: 6287252 |
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| Chan GL, Little JB, Cross-sensitivity of certain xeroderma pigmentosum and Cockayne syndrome fibroblast strains to both ionizing radiation and ultraviolet light. Mol Gen Genet181:562-3 1981 |
| PubMed ID: 6943407 |
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| Cox R, Masson WK, Weichselbaum RR, Nove J, Little JB, The repair of potentially lethal damage in x-irradiated cultures of normal and ataxia telangiectasia human fibroblasts. Int J Radiat Biol Relat Stud Phys Chem Med39:357-65 1981 |
| PubMed ID: 6971846 |
| |
| Nove J, Nichols WW, Weichselbaum RR, Little JB, Abnormalities of human chromosome 13 and in vitro radiosensitivity; a study of 19 fibroblast strains. Mutat Res84:157-67 1981 |
| PubMed ID: 7329430 |
| |
| Fornace AJ Jr, Little JB, Normal repair of DNA single-strand breaks in patients with ataxia telangiectasia. Biochim Biophys Acta607:432-7 1980 |
| PubMed ID: 6156701 |
| |
| Little JB, Nove J, Weichselbaum RR, Abnormal sensitivity of diploid skin fibroblasts from a family with gardner's syndrome to the lethal effects of X-irradiation, ultraviolet light and mitomycin-C. Mutat Res70:241-50 1980 |
| PubMed ID: 7374661 |
| |
| Weichselbaum RR, Nove J, Albert D, Little JB, An in vitro investigation of genetic susceptibility to cancer in diploid fibroblasts from retinoblastoma patients. Teratog Carcinog Mutagen1:171-9 1980 |
| PubMed ID: 6119809 |
| |
| Weichselbaum RR, Nove J, Little JB, X-ray sensitivity of fifty-three human diploid fibroblast cell strains from patients with characterized genetic disorders. Cancer Res40:920-5 1980 |
| PubMed ID: 7471105 |
| Cumulative PDL at Freeze |
16 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|