AG01521
Fibroblast from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Foreskin
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Foreskin
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XY
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
45 |
| PDL at Freeze |
12 |
| Passage Frozen |
5 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
The culture was initiated on 1/21/76 from explants of minced foreskin removed ante-mortem 1 day earlier. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Murie M, Peng Y, Rigby MJ, Dieterich IA, Farrugia MA, Endresen A, Bhattacharyya A, Puglielli L, ATase inhibition rescues age-associated proteotoxicity of the secretory pathway Communications biology5:173 2021 |
| PubMed ID: 35217767 |
| |
| Hedir S, De Giorgi M, Fogha J, De Pascale M, Weiswald LB, Brotin E, Marekha B, Denoyelle C, Denis C, Suzanne P, Gautier F, Juin P3, Ligat L, Lopez F, Carlier L, Legay R, Bureau R2, Rault S, Poulain L, Oliveira Santos JS, Voisin-Chiret AS, Structure-guided design of pyridoclax derivatives based on Noxa / Mcl-1 interaction mode European Journal of Medicinal Chemistry159:357-380 2018 |
| PubMed ID: 30308410 |
| |
| Paget V, Lechevrel M, André V, Goff JL, Pottier D, Billet S, Garçon G, Shirali P, Sichel F, Benzo[a]pyrene, aflatoxine B1 and acetaldehyde mutational patterns in TP53 gene using a functional assay: relevance to human cancer aetiology PloS one7:e30921 2011 |
| PubMed ID: 22319594 |
| |
| Wilson PF, Nagasawa H, Warner CL, Fitzek MM, Little JB, Bedford JS, Radiation Sensitivity of Primary Fibroblasts from Hereditary Retinoblastoma Family Members and Some Apparently Normal Controls: Colony Formation Ability during Continuous Low-Dose-Rate Gamma Irradiation Radiation research169:483-94 2008 |
| PubMed ID: 18439048 |
| |
| Plan Y, Hlatky L, Hahnfeldt P, Sachs R, Loucas B, Cornforth M, Full-color painting reveals an excess of radiation-induced dicentrics involving homologous chromosomes International journal of radiation biology81:613-20 2005 |
| PubMed ID: 16298942 |
| |
| Azzam EI, Nagasawa H, Yu Y, Li CY, Little JB, Cell cycle deregulation and xeroderma pigmentosum group C cell transformation. J Invest Dermatol119(6):1350-4 2002 |
| PubMed ID: 12485438 |
| |
| Azzam EI, de Toledo SM, Waker AJ, Little JB, High and low fluences of alpha-particles induce a G1 checkpoint in human diploid
fibroblasts. Cancer Res60(10):2623-31 2000 |
| PubMed ID: 10825133 |
| |
| Cleaver JE, Afzal V, Feeney L, McDowell M, Sadinski W, Volpe JP, Busch DB,
Coleman DM, Ziffer DW, Yu Y, Nagasawa H, Little JB, Increased ultraviolet sensitivity and chromosomal instability related to P53
function in the xeroderma pigmentosum variant. Cancer Res59(5):1102-8 1999 |
| PubMed ID: 10070969 |
| |
| Li CY, Nagasawa H, Dahlberg WK, Little JB, Diminished capacity for p53 in mediating a radiation-induced G1 arrest in established human tumor cell lines. Oncogene11:1885-92 1995 |
| PubMed ID: 7478618 |
| |
| Nagasawa H, Keng P, Harley R, Dahlberg W, Little JB, Relationship between gamma-ray-induced G2/M delay and cellular radiosensitivity. Int J Radiat Biol66(4):373-9 1994 |
| PubMed ID: 7930839 |
| |
| Little JB, Nagasawa H, Weichselbaum RR, Lovett DR, Schmit A, Kochenburger RJ, Blumberg PM, Differing patterns of cytotoxicity of the phorbol ester 12-O- tetradecanoylphorbol 13-acetate in various human cell strains. Carcinogenesis6:1703-8 1985 |
| PubMed ID: 3864565 |
| |
| Barfknecht TR, Little JB, Abnormal sensitivity of skin fibroblasts from familial polyposis patients to DNA alkylating agents. Cancer Res42:1249-54 1982 |
| PubMed ID: 6800649 |
| |
| Barfknecht TR, Little JB, Survival of hereditary retinoblastoma human skin fibroblasts after treatment with DNA-damaging chemicals. Mutat Res105:189-94 1982 |
| PubMed ID: 6815522 |
| |
| Barfknecht TR, Little JB, Hypersensitivity of ataxia telangiectasia skin fibroblasts to DNA alkylating agents. Mutat Res94:369-82 1982 |
| PubMed ID: 6810166 |
| |
| Fornace AJ Jr, Little JB, Normal repair of DNA single-strand breaks in patients with ataxia telangiectasia. Biochim Biophys Acta607:432-7 1980 |
| PubMed ID: 6156701 |
| PDL at Senescence |
45 |
| Cumulative PDL at Freeze |
12 |
| Passage Frozen |
5 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|