AG01519
Fibroblast from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Foreskin
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
12 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The culture was initiated on 1/21/76 from explants of minced foreskin removed ante-mortem 1 day earlier. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Tagad HD, Brito J, Marin A, Buckley C, Wang H, Sun J, Sukhishvili SA, Wang H, Andrianov AK, 4-Methylumbelliferone-Functionalized Polyphosphazene and Its Assembly into Biocompatible Fluorinated Nanocoatings with Selective Antiproliferative Activity Biomacromolecules: 2023 |
| PubMed ID: 37071718 |
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| Bergsten E, Uutela M, Li X, Pietras K, Ostman A, Heldin CH, Alitalo K, Eriksson
U, PDGF-D is a specific, protease-activated ligand for the PDGF beta-receptor. Nat Cell Biol3(5):512-6 2001 |
| PubMed ID: 11331881 |
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| Li X, Ponten A, Aase K, Karlsson L, Abramsson A, Uutela M, Backstrom G,
Hellstrom M, Bostrom H, Li H, Soriano P, Betsholtz C, Heldin CH, Alitalo K,
Ostman A, Eriksson U, PDGF-C is a new protease-activated ligand for the PDGF alpha-receptor. Nat Cell Biol2(5):302-9 2000 |
| PubMed ID: 10806482 |
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| Dahlberg, Response of dermal fibroblast cultures from patients eith unusually severe responses to radiotherapy and from ataxia telangiectasia heterozygotes to fractionated radiation. Clin Cancer Res1:785 (1995):302-9 1995 |
| PubMed ID: 10806482 |
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| Yeowell HN, Ha V, Clark WL, Marshall MK, Pinnell SR, Sequence analysis of a cDNA for lysyl hydroxylase isolated from human skin fibroblasts from a normal donor: differences from human placental lysyl hydroxylase cDNA. J Invest Dermatol102:382-4 1994 |
| PubMed ID: 8120423 |
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| Gailit J, Pierschbacher M, Clark RA, Expression of functional alpha 4 beta 1 integrin by human dermal fibroblasts. J Invest Dermatol100:323-8 1993 |
| PubMed ID: 8440915 |
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| Nagasawa H, Kraemer KH, Shiloh Y, Little JB, Detection of ataxia telangiectasia heterozygous cell lines by postirradiation cumulative labeling index: measurements with coded samples. Cancer Res47:398-402 1987 |
| PubMed ID: 3791230 |
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| Levinstone D, Eden M, Bell E, Similarity of sister-cell trajectories in fibroblast clones. J Cell Sci59:105-19 1983 |
| PubMed ID: 6863406 |
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| Marek LF, Kelley RO, A simple technique for the visualization of whole mount cytoskeletons with transmission electron microscopy. Anat Rec207:365-71 1983 |
| PubMed ID: 6359961 |
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| Lewis L, Verna JM, Levinstone D, Sher S, Marek L, Bell E, The relationship of fibroblast translocations to cell morphology and stress fibre density. J Cell Sci53:21-36 1982 |
| PubMed ID: 7045145 |
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| Marek LF, Kelley RO, Perdue BD, Organization of the cytoskeleton in square fibroblasts. Cell Motil2:115-30 1982 |
| PubMed ID: 6890876 |
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| Sarber R, Hull B, Merrill C, Soranno T, Bell E, Regulation of proliferation of fibroblasts of low and high population doubling levels grown in collagen lattices. Mech Ageing Dev17:107-17 1981 |
| PubMed ID: 7311621 |
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| Lewis, The contribution of tail snapping to fibroblast translocation. J Cell Biol87:225a (1980):107-17 1980 |
| PubMed ID: 7311621 |
| Cumulative PDL at Freeze |
12 |
| Passage Frozen |
5 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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