GM22671
LCL from B-Lymphocyte
Description:
MULTIPLE CELL LINES FROM SAME SUBJECT - EBV TRANSFORMED B LYMPHOCYTE
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family Member
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1
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Family History
|
N
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Clinically normal; other lymphoblast cell cultures from this same individual are GM22672, GM22673, GM22674 and GM22675 |
| Swafford K, Acharya B, Xu YZ, Raney T, McCrury M, Saha D, Frett B, Kendrick S, Targeting a Novel G-Quadruplex in the Genes13: 2022 |
| PubMed ID: 35885931 |
| |
| Rodden LN, Gilliam KM, Lam C, Rojsajjakul T, Mesaros C, Dionisi C, Pook M, Pandolfo M, Lynch DR, Blair IA, Bidichandani SI, DNA methylation in Friedreich ataxia silences expression of frataxin isoform E Scientific reports12:5031 2021 |
| PubMed ID: 35322126 |
| |
| Rodden LN, Chutake YK, Gilliam K, Lam C, Soragni E, Hauser L, Gilliam M, Wiley G, Anderson MP, Gottesfeld JM, Lynch DR, Bidichandani SI, Methylated and unmethylated epialleles support variegated epigenetic silencing in Friedreich ataxia Human molecular genetics29:3818-3829 2020 |
| PubMed ID: 33432325 |
| |
| Scheinfeldt LB1, Hodges K2, Pevsner J3, Berlin D2, Turan N2, Gerry NP2,4., Genetic and genomic stability across lymphoblastoid cell line expansions BMC Research Notes11:3818-3829 2018 |
| PubMed ID: 30075799 |
| |
| Shirley MD, Baugher JD, Stevens EL, Tang Z, Gerry N, Beiswanger CM, Berlin DS, Pevsner J, Chromosomal variation in lymphoblastoid cell lines Human mutation33:1075-86 2011 |
| PubMed ID: 22374857 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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