GM21808
Fibroblast from Skin, Foreskin
Description:
MULTIPLE CELL TYPES FROM SAME SUBJECT - FORESKIN
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Foreskin
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Race
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White
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Family Member
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1
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
7.17 |
| Passage Frozen |
4 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
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| Remarks |
Foreskin fibroblast cell culture; other cultures from this same donor are GM21810 (melanocyte) and GM21806 (keratinocyte) |
| Tucker MH, Yu W, Menden HL, Xia S, Schreck CF, Gibson MI, Louiselle DA, Pastinen T, Raje N, Sampath V, IRF7 and UNC93B1 variants in an infant with recurrent herpes simplex virus infection The Journal of clinical investigation: 2023 |
| PubMed ID: 37097753 |
| |
| Cotticelli MG, Xia S, Truitt R, Doliba NM, Rozo AV, Tobias JW, Lee T, Chen J, Napierala JS, Napierala M, Yang W, Wilson RB, Acute frataxin knockdown in induced pluripotent stem cell-derived cardiomyocytes activates a type I interferon response Disease models & mechanisms16: 2022 |
| PubMed ID: 36107856 |
| |
| Napierala JS, Rajapakshe K, Clark A, Chen YY, Huang S, Mesaros C, Xu P, Blair IA, Hauser LA, Farmer J, Lynch DR, Edwards DP, Coarfa C, Napierala M, Reverse phase protein array reveals correlation of retinoic acid metabolism with cardiomyopathy in Friedreich's ataxia Molecular & cellular proteomics : MCP16:100094 2021 |
| PubMed ID: 33991687 |
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| Gebauer JM, Flachsenberg F, Windler C, Richer B, Baumann U, Seeger K, Structural and biophysical characterization of the type VII collagen vWFA2 subdomain leads to identification of two binding sites FEBS open bio16:100094 2019 |
| PubMed ID: 32031736 |
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| Napierala JS, Li Y, Lu Y, Lin K, Hauser LA, Lynch DR, Napierala M, Comprehensive analysis of gene expression patterns in Friedreich's ataxia fibroblasts by RNA sequencing reveals altered levels of protein synthesis factors and solute carriers Disease models & mechanisms10:1353-1369 2017 |
| PubMed ID: 29125828 |
| Cumulative PDL at Freeze |
8.76 |
| Passage Frozen |
4 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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