GM14217
LCL from B-Lymphocyte
Description:
CHROMOSOME INSERTION
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX,ins(5;15)(5pter>5q23.2::15q22> 15q25::5q23.2>5qter;15pter>15q22:: 15q25>15qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Cytogenetics |
Chromosome 15: INSERTION Breakpoint 15q22 ins(5;15)15q22 |
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Chromosome 15: INSERTION Breakpoint 15q25 ins(5;15)15q25 |
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Chromosome 5: INSERTION Breakpoint 5q23 ins(5;15)5q23 |
| Remarks |
Line 1111; ins(5;15)(q23.2;q22q25) |
| Loftus SK, Shiang R, Warrington JA, Bengtsson U, McPherson JD, Wasmuth JJ, Genes encoding adrenergic receptors are not clustered on the long arm of human chromosome 5. Cytogenet Cell Genet67:69-74 1994 |
| PubMed ID: 8039425 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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