GM11511
LCL from B-Lymphocyte
Description:
CHROMOSOME INSERTION
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family Member
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2
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Relation to Proband
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father
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XY,ins(4;14)(4pter>4q13.2::14q13.3>14q23.3::4q13,2>4qter;14pter>14q13.3::14q23.3>14qter).ish ins(4;14)(wcp4+,wcp14+;wcp4-,wcp14+)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Phenotypically normal; 46,XY,ins(4;14) (q13.2;q13.3q23.3).ish ins(4;14)(wcp4+, wcp14+;wcp4-,wcp14+) |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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