Description:
RING CHROMOSOME
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Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Chromosome Abnormalities |
|
Cell Type
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Fibroblast
|
|
Transformant
|
Untransformed
|
|
Race
|
White
|
|
Relation to Proband
|
proband
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|
Confirmation
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Karyotypic analysis after cell line submission to CCR
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|
ISCN
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46,XY,r(13)
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|
Species
|
Homo sapiens
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|
Common Name
|
Human
|
|
Remarks
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|
| Passage Frozen |
3 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
Breakpoint may be q14; severely malformed fetus; anencephaly, imperforate anus, urethral meatus, severe talipes, syndactyly, horseshoe cataracts, bilateral retinal dysplasia, and type IV polycystic kidneys |
| Passage Frozen |
3 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|