AG29403

AG29403 iPSC from Fibroblast

Description:

CENTENARIAN STUDY CENTENARIAN

Affected:

No

Sex:

Female

Age:

100 YR (At Sampling)

Overview

Repository

NIA Aging Cell Culture Repository

Subcollection

Apparently healthy iPSCs
University of Georgia Centenarian Study

Protocols

Protocol PDF

Biopsy Source

Skin

Cell Type

Stem cell

Cell Subtype

Induced pluripotent stem cell

Transformant

Reprogrammed (Sendai)

Sample Source

iPSC from Fibroblast

Race

Black/African American

Relation to Proband

proband

Confirmation

Clinical summary/Case history

ISCN

46,XX[19]

Species

Homo sapiens

Common Name

Human

Remarks

Reprogrammed from parental fibroblast AG19459; clinically normal; age verified. Researchers purchasing hiPSCs from the NIA Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is Sendai-CytoTune.

Characterizations

Passage Frozen

Induced Pluripotent Stem Cell

The parental cell line was recovered reprogrammed to an induced pluripotent stem cell line and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

Phenotypic Data

Remarks

Culture Protocols

Passage Frozen

Split Ratio

1:9

Temperature

37 C

Percent CO2

Percent O2

AMBIENT

Medium

mTeSR1

Serum

0% none

Substrate

Matrigel

Supplement

Pricing

Commercial:

$1,131.00USD

Academic &
Non-profit:

$823.00USD

NIA Grantees:

$412.00USD