GM10908
Fibroblast from Gonad, Ovary
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Ovary
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Cell Type
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Fibroblast
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Tissue Type
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Gonad
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Gonad, Ovary
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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Verified as Chinese hamster with abnormal chromosomes; 68% of cells are diploid & 32% are tetraploid
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Chinese hamster ovary line UV-135; sensitive to UV irradiation; UV repair mutation can be complemented by a gene, ERCC5, on human chromosome #13; 68% of cells are diploid & 32% are tetraploid; fibroblast culture |
| Thorner PS, Zheng K, Kalluri R, Jacobs R, Hudson BG, Coordinate gene expression of the alpha3, alpha4, and alpha5 chains of collagen type IV. Evidence from a canine model of X-linked nephritis with a COL4A5 gene mutation. J Biol Chem271(23):13821-8 1996 |
| PubMed ID: 8662866 |
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| Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
| PubMed ID: 8478002 |
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| Magovcevic I, Ang SL, Seidman JG, Tolman CJ, Neer EJ, Morton CC, Regional localization of the human G protein alpha i2 (GNAI2) gene: assignment to 3p21 and a related sequence (GNAI2L) to 12p12-p13. Genomics12:125-9 1992 |
| PubMed ID: 1733849 |
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| Thompson LH, Carrano AV, Sato K, Salazar EP, White BF, Stewart SA, Minkler JL, Siciliano MJ, Identification of nucleotide-excision-repair genes on human chromosomes 2 and 13 by functional complementation in hamster-human hybrids. Somat Cell Mol Genet13:539-51 1987 |
| PubMed ID: 3477874 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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