AG08525
Fibroblast from Skin, Arm
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
German Alzheimer Disease |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Ethnicity
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GERMAN
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Family Member
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2
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Relation to Proband
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IV-22
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.02 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
The donor is an unaffected spouse of an Alzheimer's disease family member. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 11/05/85 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 3. A lymphoblast culture from same donor is AG08524. |
| Zhou LT, Liu D, Kang HC, Lu L, Huang HZ, Ai WQ, Zhou Y, Deng MF, Li H, Liu ZQ, Zhang WF, Hu YZ, Han ZT, Zhang HH, Jia JJ, Sarkar AK, Sharaydeh S, Wang J, Man HY, Schilling M, Bertram L, Lu Y, Guo Z, Zhu LQ, Tau pathology epigenetically remodels the neuron-glial cross-talk in Alzheimer's disease Science advances9:eabq7105 2023 |
| PubMed ID: 37083538 |
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| Rossi A, Rigotto G, Valente G, Giorgio V, Basso E, Filadi R, Pizzo P, Defective Mitochondrial Pyruvate Flux Affects Cell Bioenergetics in Alzheimer's Disease-Related Models Cell reports30:2332-2348.e10 2019 |
| PubMed ID: 32075767 |
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| Greotti E, Capitanio P, Wong A, Pozzan T, Pizzo P, Pendin D, Familial Alzheimer's disease-linked presenilin mutants and intracellular Ca Cell calcium79:44-56 2018 |
| PubMed ID: 30822648 |
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| Frommelt P, Schnabel R, Kuhne W, Nee LE, Polinsky RJ, Familial Alzheimer disease: a large, multigeneration German kindred. Alzheimer Dis Assoc Disord5:36-43 1991 |
| PubMed ID: 2025423 |
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| St George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Myers RH, Feldman RG, Pollen D, Drachman D, et al, The genetic defect causing familial Alzheimer's disease maps on chromosome 21. Science235:885-90 1987 |
| PubMed ID: 2880399 |
| Cumulative PDL at Freeze |
8 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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