Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 12 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
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No Data
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Subcollection |
Hybrids |
Sample Source
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No Data
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Subject Type
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No Data
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Family Type
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No Data
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Ethnicity
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No Data
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Family Member
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No Data
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Genetic Data
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No Data
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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Callen DF, Baker E, Eyre HJ, Lane SA, An expanded mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet33:190-5 1990 |
PubMed ID: 2095701 |
Split Ratio |
1:2 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Adenine 5 x 10-2mM |
Supplement |
Azaserine 5 x 10-2mM |
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