Description:
CHROMOSOME 4 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
|
No Data
|
Subcollection |
Hybrids |
Sample Source
|
No Data
|
Subject Type
|
No Data
|
Family Type
|
No Data
|
Ethnicity
|
No Data
|
Family Member
|
No Data
|
Genetic Data
|
No Data
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Wijmenga C, Winokur ST, Padberg GW, Skraastad MI, Altherr MR, Wasmuth JJ, Murray JC, Hofker MH, Frants RR, The human skeletal muscle adenine nucleotide translocator gene maps to chromosome 4q35 in the region of the facioscapulohumeral muscular dystrophy locus. Hum Genet92:198-203 1993 |
PubMed ID: 8103757 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Aminopterin 4 x 10-7M |
Supplement |
Hypoxanthine 1 x 10-4M |
Supplement |
Thymidine 1.6 x 10-5M |
|
|