Description:
CHROMOSOME 4 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
|
No Data
|
Subcollection |
Hybrids |
Sample Source
|
No Data
|
Subject Type
|
No Data
|
Family Type
|
No Data
|
Ethnicity
|
No Data
|
Family Member
|
No Data
|
Genetic Data
|
No Data
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 9q22.3-q31, D9S53, and gave positive results with primers for 9p24, WI-7297, and 9q34-qter, D9S158. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 19p13.2, LDLR. |
|
Hayden MR, Hewitt J, Wasmuth JJ, Kastelein JJ, Langlois S, Conneally M, Haines J, Smith B, Hilbert C, Allard D, A polymorphic DNA marker that represents a conserved expressed sequence in the region of the Huntington disease gene. Am J Hum Genet42:125-31 1988 |
PubMed ID: 2892395 |
|
Smith B, Skarecky D, Bengtsson U, Magenis RE, Carpenter N, Wasmuth JJ, Isolation of DNA markers in the direction of the Huntington disease gene from the G8 locus. Am J Hum Genet42:335-44 1988 |
PubMed ID: 2963539 |
Split Ratio |
1:5 |
Temperature |
39 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Proline 34.5mg/L |
|
|