Description:
CHROMOSOME 6 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 7 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
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No Data
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Subcollection |
Hybrids |
Sample Source
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No Data
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Subject Type
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No Data
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Family Type
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No Data
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Ethnicity
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No Data
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Family Member
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No Data
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Genetic Data
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No Data
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6p25, D6S344, and gave positive results with primers for 6q21-q23.3, D6S292, 6q24-q27, MAS1, and 6q27-qter, D6S297. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 7p12, EGFR, and 7pter-p22, D7S472, and gave negative results with primers for 7q31.3, CFTR, and 7q36-qter, D7S550. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 10q24, PLAU. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 22q11.2, IGLC2. |
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Pappas GJ, Polymeropoulos MH, Boyle JM, Trent JM, Regional assignment by hybrid mapping of 36 expressed sequence tags (ESTs) on human chromosome 6. Genomics25:124-9 1995 |
PubMed ID: 7774909 |
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Meese EU, Meltzer PS, Ferguson PW, Trent JM, Alu-PCR: characterization of a chromosome 6-specific hybrid mapping panel and cloning of chromosome-specific markers. Genomics12:549-54 1992 |
PubMed ID: 1559706 |
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Meese EU, Witkowski CM, Zoghbi HY, Stanbridge EJ, Meltzer PS, Trent JM, Development and utilization of a somatic cell hybrid mapping panel to assign NotI linking probes to the long arm of human chromosome 6. Genomics12:542-8 1992 |
PubMed ID: 1559705 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Aminopterin 4 x 10-7M |
Supplement |
Hypoxanthine 1 x 10-4M |
Supplement |
Thymidine 1.6 x 10-5M |
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