Description:
CHROMOSOME 8 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
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No Data
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Subcollection |
Hybrids |
Sample Source
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No Data
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Subject Type
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No Data
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Family Type
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No Data
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Ethnicity
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No Data
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Family Member
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No Data
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Genetic Data
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No Data
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
M. Wagner, Research Triangle Park, NC, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p21.3-cen, D8S259, 8q11, MOS, and 8q13-q22.1, D8S286, and gave negative results with primers for 8p22, D8S258, 8q22.2, D8S271, 8q22.3, D8S276, and 8q24.3-qter, D8S263. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p12, D8S87, 8p21-p11.2, LHRH, and 8q11.22-q12, D8S260, and gave negative results with primers for 8p23-p22, DEF1, 8q23-q24, D8S199, 8q24, TG, and 8q22.2, D8S270. |
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Wagner MJ, Ge Y, Siciliano M, Wells DE, A hybrid cell mapping panel for regional localization of probes to human chromosome 8. Genomics10:114-25 1991 |
PubMed ID: 2045096 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
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