Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
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No Data
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Subcollection |
Hybrids |
Sample Source
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No Data
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Subject Type
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No Data
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Family Type
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No Data
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Ethnicity
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No Data
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Family Member
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No Data
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Genetic Data
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No Data
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 11p13, CAT, and gave a negative result with a primer for 11q21-q22, CLG. |
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GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 16p13.3, HBA, and gave positive results with primers for 16q22.1, LCAT, 16q23.2, D16S266, 16q24.2, D16S389, 16q24.3, D16S305, and 16q24.3, PRSM1. |
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Callen DF, Doggett NA, Stallings RL, Chen LZ, Whitmore SA, Lane SA, Nancarrow JK, Apostolou S, Thompson AD, Lapsys NM, et al, High-resolution cytogenetic-based physical map of human chromosome 16. Genomics13:1178-85 1992 |
PubMed ID: 1505951 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Adenine 5 x 10-2mM |
Supplement |
Azaserine 5 x 10-2mM |
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