Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE Y CHROMOSOME SOMATIC CELL HYBRIDS
Repository
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No Data
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Subcollection |
Hybrids |
Sample Source
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No Data
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Subject Type
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No Data
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Family Type
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No Data
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Ethnicity
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No Data
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Family Member
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No Data
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Genetic Data
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No Data
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
PubMed ID: 12829847 |
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Van Keuren ML, Watkins PC, Drabkin HA, Jabs EW, Gusella JF, Patterson D, Regional localization of DNA sequences on chromosome 21 using somatic cell hybrids. Am J Hum Genet38:793-804 1986 |
PubMed ID: 3014865 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Ham's F12 deficient Medium with 2mM L-glutamine or equivalent |
Serum |
5% dialyzed fetal bovine serum |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Proline 50mg/ml stock |
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