Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently healthy iPSCs
Apparently Healthy Collection
Protocols Protocol PDF
Biopsy Source Arm
Cell Type Stem cell
Tissue Type Induced pluripotent stem cell
Transformant Reprogrammed (Retroviral)
Race Caucasian
Hispanic Ethnicity Not Hispanic/Latino
Country of Origin USA
Family Member 1
Family History N
Relation to Proband proband
ISCN 46,XY.arr[hg19](1-22)x2,(XY)x1
Species Homo sapiens
Common Name Human
Remarks Apparently healthy individual (J. Craig Venter); induced pluripotent stem cell line derived from retroviral reprogramming of fibroblast cells (skin, arm); parental fibroblast not in repository; see NS12911 for DNA; for more information, go to: https://catalog.coriell.org/1/HuRef. This iPSC line, CV-hiPSC-B or CVB, was submitted by Dr. Lawrence Goldstein (University of California San Diego).
Passage Frozen 20
 
Induced Pluripotent Stem Cell The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.
 
Remark Apparently healthy individual (J. Craig Venter); induced pluripotent stem cell line derived from retroviral reprogramming of fibroblast cells (skin, arm); parental fibroblast not in repository; see NS12911 for DNA; for more information, go to: https://catalog.coriell.org/1/HuRef. This iPSC line, CV-hiPSC-B or CVB, was submitted by Dr. Lawrence Goldstein (University of California San Diego).
Jain M, Olsen HE, Turner DJ, Stoddart D, Bulazel KV, Paten B, Haussler D, Willard HF, Akeson M, Miga KH, Linear assembly of a human centromere on the Y chromosome Nature biotechnology36:321-323 2017
PubMed ID: 29553574
 
Woodruff G, Young JE, Martinez FJ, Buen F, Gore A, Kinaga J, Li Z, Yuan SH, Zhang K, Goldstein LS, The presenilin-1 ?E9 mutation results in reduced ?-secretase activity, but not total loss of PS1 function, in isogenic human stem cells Cell reports5:974-85 2013
PubMed ID: 24239350
 
Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K, Somatic coding mutations in human induced pluripotent stem cells Nature471:63-7 2010
PubMed ID: 21368825
No data is available
Cumulative PDL at Freeze 3
Passage Frozen 20
Split Ratio 1:6
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium HES/Human ES Cell Medium
Serum 20% Knock-out Serum Replacement Not inactivated
Substrate Gelatin + Feeder Layer