Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
PIGI Consented Sample
Quantity 50 µg
Quantitation Method Please see our FAQ
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Asian, Other
Ethnicity Chinese
Country of Origin USA
Family Member 1
Family History N
Relation to Proband proband
ISCN 46,XY.arr[hg19](1-22)x2,(XY)x1
Species Homo sapiens
Common Name Human
Remarks Participant (hu91BD69) in the Personal Genome Project: http://www.personalgenomes.org history of: eczema; lactose intolerance; nearsightedness; same subject as GM26107 (stem cell); father is GM24694 (Lymph); mother is GM24695 (Lymph).
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by LINE assay
 
Remark Participant (hu91BD69) in the Personal Genome Project: http://www.personalgenomes.org history of: eczema; lactose intolerance; nearsightedness; same subject as GM26107 (stem cell); father is GM24694 (Lymph); mother is GM24695 (Lymph).
Atkins A, Gupta P, Zhang BM, Tsai WS, Lucas J, Javey M, Vora A, Mei R, Detection of Circulating Tumor DNA with a Single-Molecule Sequencing Analysis Validated for Targeted and Immunotherapy Selection Molecular diagnosis & therapy: 2019
PubMed ID: 31209714
 
Cleveland MH1, Zook JM2, Salit M3, Vallone PM2., Determining Performance Metrics for Targeted Next-Generation Sequencing Panels Using Reference Materials Journal of Molecular Diagnostics18:1525-1578 2018
PubMed ID: 29959024
 
Lincoln SE, Truty R, Lin CF, Zook JM, Paul J, Ramey VH, Salit M, Rehm HL, Nussbaum RL, Lebo MS, A Rigorous Interlaboratory Examination of the Need to Confirm Next-Generation Sequencing-Detected Variants with an Orthogonal Method in Clinical Genetic Testing The Journal of molecular diagnostics : JMD18:1525-1578 2018
PubMed ID: 30610921
 
Soukupova J, Zemankova P, Lhotova K, Janatova M, Borecka M, Stolarova L, Lhota F, Foretova L, Machackova E, Stranecky V, Tavandzis S, Kleiblova P, Vocka M, Hartmannova H, Hodanova K, Kmoch S, Kleibl Z, Validation of CZECANCA (CZEch CAncer paNel for Clinical Application) for targeted NGS-based analysis of hereditary cancer syndromes PloS one13:e0195761 2017
PubMed ID: 29649263
 
Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016
PubMed ID: 27724973
 
Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005
PubMed ID: 16729065
No data is available
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium