Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Apparently healthy iPSCs
Protocols Protocol PDF
Biopsy Source Lung
Cell Type Stem cell
Tissue Type Induced pluripotent stem cell
Transformant Reprogrammed (Retroviral)
Race Black
Family Member 1
Relation to Proband proband
Confirmation Karyotypic analysis and Case history
ISCN 46,XX
Species Homo sapiens
Common Name Human
Remarks iPS cell line derived from lung fibroblast GM06112; same patient as GM06111; 46,XX; 4% of cells show random chromosome loss.
Passage Frozen 27
 
Induced Pluripotent Stem Cell The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and directed differentiation toward cardiac neuronal and pancreatic lineages. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. The line was evaluated for in vivo pluripotency via teratoma formation assay. Characterization data are included in the Certificate of Analysis.
 
Remark iPS cell line derived from lung fibroblast GM06112; same patient as GM06111; 46,XX; 4% of cells show random chromosome loss.
No data is available
No data is available
Passage Frozen 27
Split Ratio 1:1
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 20% Knock-out Serum Replacement
Substrate Gelatin + Feeder Layer