Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently healthy iPSCs
Apparently Healthy Collection
Protocols Protocol PDF
Biopsy Source Skin
Cell Type Stem cell
Tissue Type Induced pluripotent stem cell
Transformant Reprogrammed (Retroviral)
Race Black
Family Member 1
Relation to Proband proband
Confirmation Karyotypic analysis and Case history
ISCN 47,XXX[2]/46,XX[23]
Species Homo sapiens
Common Name Human
Remarks iPS line from skin fibroblast culture GM06111; 46,XX; 6% of cells show random chromosome loss.
Induced Pluripotent Stem Cell The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.
 
Remark iPS line from skin fibroblast culture GM06111; 46,XX; 6% of cells show random chromosome loss.
No data is available
No data is available
Split Ratio 1:6
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 20% Knock-out Serum Replacement Not inactivated
Substrate Gelatin + Feeder Layer
Supplement Basic Fibroblast Growth Factor 100ng/ml