Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently healthy iPSCs
Apparently Healthy Collection
Protocols Protocol PDF
Biopsy Source Skin
Cell Type Stem cell
Tissue Type Induced pluripotent stem cell
Transformant Reprogrammed (Retroviral)
Race Caucasian
Family Member 1
Family History N
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XX[25]
Species Homo sapiens
Common Name Human
Remarks Induced pluripotent stem cell line made from facial dermal fibroblasts; retroviruses used contained human Oct3/4, Sox2, Klf4, and c-Myc. Prior to ordering this iPSC line, for-profit institutions must contact iPS Academia Japan, Inc. to obtain a license: iPS Academia Japan, Inc. 448-5-201, Kajii-cho, Imadegawa Kawaramachi, Kamigyo-ku Kyoto-shi, Kyoto, 602-0841, JAPAN Attn.: License Department Phone: +81-75-256-8582, Fax: +81-75-256-6211 E-mail: license@ips-ac.co.jp
Passage Frozen 23
 
Induced Pluripotent Stem Cell The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and directed differentiation toward cardiac neuronal and pancreatic lineages. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. The line was evaluated for in vivo pluripotency via teratoma formation assay. Characterization data are included in the Certificate of Analysis.
 
Remark Induced pluripotent stem cell line made from facial dermal fibroblasts; retroviruses used contained human Oct3/4, Sox2, Klf4, and c-Myc. Prior to ordering this iPSC line, for-profit institutions must contact iPS Academia Japan, Inc. to obtain a license: iPS Academia Japan, Inc. 448-5-201, Kajii-cho, Imadegawa Kawaramachi, Kamigyo-ku Kyoto-shi, Kyoto, 602-0841, JAPAN Attn.: License Department Phone: +81-75-256-8582, Fax: +81-75-256-6211 E-mail: license@ips-ac.co.jp
Goto-Silva L, Ayad NME, Herzog IL, Silva NP, Lamien B, Orlande HRB, da Costa Souza A, Ribeiro S, Martins M, Domont GB, Junqueira M, Tovar-Moll F, Rehen SK, Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms BMC developmental biology19:3 2018
PubMed ID: 30841924
 
Casas BS, Vitória G, do Costa MN, Madeiro da Costa R, Trindade P, Maciel R, Navarrete N, Rehen SK, Palma V, hiPSC-derived neural stem cells from patients with schizophrenia induce an impaired angiogenesis Translational psychiatry8:48 2017
PubMed ID: 29467462
 
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S., Induction of pluripotent stem cells from adult human fibroblasts by defined factors Cell131:861-872 2007
PubMed ID: 18035408
No data is available
Passage Frozen 23
Split Ratio 1:6
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 20% Knock-out Serum Replacement Not inactivated
Substrate Gelatin + Feeder Layer