Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Biopsy Source Foreskin
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Race Caucasian
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XY
Species Homo sapiens
Common Name Human
Remarks Foreskin fibroblast cell culture; other cultures from this same donor are GM22134 (melanocyte) and GM22135 (keratinocyte)
PDL at Senescence 29
PDL at Freeze 4.52
Passage Frozen 1
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin confirmed by LINE assay
 
Remark Foreskin fibroblast cell culture; other cultures from this same donor are GM22134 (melanocyte) and GM22135 (keratinocyte)
No data is available
No data is available
PDL at Senescence 29
Cumulative PDL at Freeze 4.52
Passage Frozen 1
Split Ratio 1:20
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Substrate Commercially-treated plastic