Coriell Institute
Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Biopsy Source Foreskin
Cell Type Keratinocyte
Tissue Type Skin
Transformant Untransformed
Race Caucasian
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XY
Species Homo sapiens
Common Name Human
Remarks Foreskin keratinocyte cell culture; this culture tested 95% positive for keratinocyte cell-specific cytokeratin (immunocytochemistry assay); other cultures from this same donor are GM22136 (fibroblast) and GM22134 (melanocyte). Must ship frozen.
PDL at Senescence 38
PDL at Freeze 3.56
Passage Frozen 1
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin confirmed by LINE assay
 
Remark Foreskin keratinocyte cell culture; this culture tested 95% positive for keratinocyte cell-specific cytokeratin (immunocytochemistry assay); other cultures from this same donor are GM22136 (fibroblast) and GM22134 (melanocyte). Must ship frozen.
Shirley MD, Baugher JD, Stevens EL, Tang Z, Gerry N, Beiswanger CM, Berlin DS, Pevsner J, Chromosomal variation in lymphoblastoid cell lines Human mutation33:1075-86 2011
PubMed ID: 22374857
No data is available
PDL at Senescence 38
Cumulative PDL at Freeze 3.56
Passage Frozen 1
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium CnT-07 Epidermal Progenitor Cell Medium
Serum none
Substrate Collagen IV-coated

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