Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Biopsy Source Foreskin
Cell Type Melanocyte
Tissue Type Skin
Transformant Untransformed
Race Caucasian
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XY
Species Homo sapiens
Common Name Human
Remarks Foreskin melanocyte cell culture; this culture tested 99% positive for melanocyte cell-specific gp100 (immunocytochemistry assay); other cultures from this same donor are GM22136 (fibroblast) and GM22135 (keratinocyte). Must ship frozen.
PDL at Senescence 26
PDL at Freeze 3.66
Passage Frozen 1
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin confirmed by LINE assay
 
Remark Foreskin melanocyte cell culture; this culture tested 99% positive for melanocyte cell-specific gp100 (immunocytochemistry assay); other cultures from this same donor are GM22136 (fibroblast) and GM22135 (keratinocyte). Must ship frozen.
Shirley MD, Baugher JD, Stevens EL, Tang Z, Gerry N, Beiswanger CM, Berlin DS, Pevsner J, Chromosomal variation in lymphoblastoid cell lines Human mutation33:1075-86 2011
PubMed ID: 22374857
No data is available
PDL at Senescence 26
Cumulative PDL at Freeze 3.66
Passage Frozen 1
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Melanocyte Growth Medium
Serum 0% fetal bovine serum Not inactivated
Substrate Collagen IV-coated