Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Quantity 50 µg
Quantitation Method Please see our FAQ
Biopsy Source Lung
Cell Type Fibroblast
Tissue Type Lung
Transformant Untransformed
Race Black
Family Member 1
Relation to Proband proband
Confirmation Karyotypic analysis and Case history
ISCN arr(1-22)x2,(XY)x1
Species Homo sapiens
Common Name Human
Remarks Lung biopsy; same patient as GM06113; 46,XY; 12% of cells show random chromosome loss.
Passage Frozen 3
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
Remark Lung biopsy; same patient as GM06113; 46,XY; 12% of cells show random chromosome loss.
Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013
PubMed ID: 23665875
Sabatini F, Petecchia L, Boero S, Silvestri M, Klar J, Tenor H, Beume R, Hatzelmann A, Rossi GA, A phosphodiesterase 4 inhibitor, roflumilast N-oxide, inhibits human lung fibroblast functions in vitro Pulmonary pharmacology & therapeutics23:283-91 2009
PubMed ID: 20226872
Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995
PubMed ID: 7534307
No data is available
Passage Frozen 3
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA